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Title: Field-scale evaluation of in situ bioaugmentation for remediation of chlorinated solvents in groundwater

Abstract

Groundwater contaminated with 1,000--2,500 {micro}g/L chlorinated ethenes (trichloroethene [TCE], dichloroethene [DCE], vinyl chloride [VC]) was treated by in situ bioaugmentation with a specialized microorganisms, Burkholderia cepacia ENV435. The strain was selected for its limited adhesion to aquifer solids and its ability to degrade chlorinated ethenes in the absence of inducing cosubstrates. Approximately 550 L of the toluene ortho-monooxygenase-producing ENV435 culture ({approximately} 100 g/L) was injected along with oxygen (20 mg/L) into a semi-confined silty-sand aquifer (test plot). An equal volume of basal salts medium was simultaneously injected into an adjacent control plot. The plots each measured 4.6 m wide by 12 m long, were spaced 9.2 m apart and contained 18 multilevel monitoring wells. Groundwater ENV 435 concentrations exceeded 1 {times} 10{sup 8} cfu/mL 7 days after injection, and the strain traveled the 12 m from injection to recovery well with an average linear velocity of 0.37 to 0.54 m/day. By comparison, a bromide tracer passed through the same formation at a rate of 0.53 to 0.68 m/day. In one trial, the total mass of TCE, DCE, and VC in the treated area was reduced by as much as 78% within 2 days after injecting the organisms.

Authors:
; ; ; ;  [1]
  1. Envirogen, Inc., Lawrenceville, NJ (United States)
Publication Date:
OSTI Identifier:
687395
Resource Type:
Journal Article
Journal Name:
Environmental Science and Technology
Additional Journal Information:
Journal Volume: 33; Journal Issue: 16; Other Information: PBD: 15 Aug 1999
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; REMEDIAL ACTION; GROUND WATER; IN-SITU PROCESSING; BIODEGRADATION; CHLORINATED ALIPHATIC HYDROCARBONS

Citation Formats

Steffan, R.J., Sperry, K.L., Walsh, M.T., Vainberg, S., and Condee, C.W. Field-scale evaluation of in situ bioaugmentation for remediation of chlorinated solvents in groundwater. United States: N. p., 1999. Web. doi:10.1021/es9812204.
Steffan, R.J., Sperry, K.L., Walsh, M.T., Vainberg, S., & Condee, C.W. Field-scale evaluation of in situ bioaugmentation for remediation of chlorinated solvents in groundwater. United States. doi:10.1021/es9812204.
Steffan, R.J., Sperry, K.L., Walsh, M.T., Vainberg, S., and Condee, C.W. Sun . "Field-scale evaluation of in situ bioaugmentation for remediation of chlorinated solvents in groundwater". United States. doi:10.1021/es9812204.
@article{osti_687395,
title = {Field-scale evaluation of in situ bioaugmentation for remediation of chlorinated solvents in groundwater},
author = {Steffan, R.J. and Sperry, K.L. and Walsh, M.T. and Vainberg, S. and Condee, C.W.},
abstractNote = {Groundwater contaminated with 1,000--2,500 {micro}g/L chlorinated ethenes (trichloroethene [TCE], dichloroethene [DCE], vinyl chloride [VC]) was treated by in situ bioaugmentation with a specialized microorganisms, Burkholderia cepacia ENV435. The strain was selected for its limited adhesion to aquifer solids and its ability to degrade chlorinated ethenes in the absence of inducing cosubstrates. Approximately 550 L of the toluene ortho-monooxygenase-producing ENV435 culture ({approximately} 100 g/L) was injected along with oxygen (20 mg/L) into a semi-confined silty-sand aquifer (test plot). An equal volume of basal salts medium was simultaneously injected into an adjacent control plot. The plots each measured 4.6 m wide by 12 m long, were spaced 9.2 m apart and contained 18 multilevel monitoring wells. Groundwater ENV 435 concentrations exceeded 1 {times} 10{sup 8} cfu/mL 7 days after injection, and the strain traveled the 12 m from injection to recovery well with an average linear velocity of 0.37 to 0.54 m/day. By comparison, a bromide tracer passed through the same formation at a rate of 0.53 to 0.68 m/day. In one trial, the total mass of TCE, DCE, and VC in the treated area was reduced by as much as 78% within 2 days after injecting the organisms.},
doi = {10.1021/es9812204},
journal = {Environmental Science and Technology},
number = 16,
volume = 33,
place = {United States},
year = {1999},
month = {8}
}