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Title: Hydroxylated PAHs in bile of deep-sea fish. Relationship with xenobiotic metabolizing enzymes

Abstract

Polycyclic aromatic hydrocarbon (PAH) pollution in deep-sea environments has been assessed by measuring bile PAH metabolites in deep-sea fish. Five species from the NW Mediterranean were selected for the study: Coryphaenoides guentheri, Lepidion lepidion, Mora moro, Bathypterois mediterraneus, and Alepocephalus rostratus. Bile crude samples were directly analyzed by HPLC-fluorescence at the excitation/emission wavelengths of benzo[a]pyrene. Differences among sampling sites were recorded, which suggests that coastal discharges of contaminants may reach these remote areas. Subsequently, a number of bile samples were hydrolyzed and analyzed by gas chromatography--mass spectrometry (GC-MS) for the determination of individual PAHs. 1-Pyrenol and 2-phenylphenol were among the most abundant compounds detected. The results obtained confirm the long-range transport of PAHs to deep-sea environments, subsequent exposure of fish inhabiting those remote areas, and its ability to metabolize and excrete them through the bile. The data also describe hepatic enzymes (cytochrome P450 and glutathione S-transferases) that appear to be as catalytically efficient as those in shallow water species.

Authors:
;  [1]
  1. IIQAB-CSIC, Barcelona (Spain). Environmental Chemistry Dept.
Publication Date:
OSTI Identifier:
687387
Resource Type:
Journal Article
Journal Name:
Environmental Science and Technology
Additional Journal Information:
Journal Volume: 33; Journal Issue: 16; Other Information: PBD: 15 Aug 1999
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 56 BIOLOGY AND MEDICINE, APPLIED STUDIES; WATER POLLUTION; MEDITERRANEAN SEA; FOOD CHAINS; POLYCYCLIC AROMATIC HYDROCARBONS; BIOLOGICAL ACCUMULATION; CHEMICAL ANALYSIS; LONG-RANGE TRANSPORT; METABOLISM; HYDROXYLASES; TRANSFERASES

Citation Formats

Escartin, E., and Porte, C. Hydroxylated PAHs in bile of deep-sea fish. Relationship with xenobiotic metabolizing enzymes. United States: N. p., 1999. Web. doi:10.1021/es9902322.
Escartin, E., & Porte, C. Hydroxylated PAHs in bile of deep-sea fish. Relationship with xenobiotic metabolizing enzymes. United States. doi:10.1021/es9902322.
Escartin, E., and Porte, C. Sun . "Hydroxylated PAHs in bile of deep-sea fish. Relationship with xenobiotic metabolizing enzymes". United States. doi:10.1021/es9902322.
@article{osti_687387,
title = {Hydroxylated PAHs in bile of deep-sea fish. Relationship with xenobiotic metabolizing enzymes},
author = {Escartin, E. and Porte, C.},
abstractNote = {Polycyclic aromatic hydrocarbon (PAH) pollution in deep-sea environments has been assessed by measuring bile PAH metabolites in deep-sea fish. Five species from the NW Mediterranean were selected for the study: Coryphaenoides guentheri, Lepidion lepidion, Mora moro, Bathypterois mediterraneus, and Alepocephalus rostratus. Bile crude samples were directly analyzed by HPLC-fluorescence at the excitation/emission wavelengths of benzo[a]pyrene. Differences among sampling sites were recorded, which suggests that coastal discharges of contaminants may reach these remote areas. Subsequently, a number of bile samples were hydrolyzed and analyzed by gas chromatography--mass spectrometry (GC-MS) for the determination of individual PAHs. 1-Pyrenol and 2-phenylphenol were among the most abundant compounds detected. The results obtained confirm the long-range transport of PAHs to deep-sea environments, subsequent exposure of fish inhabiting those remote areas, and its ability to metabolize and excrete them through the bile. The data also describe hepatic enzymes (cytochrome P450 and glutathione S-transferases) that appear to be as catalytically efficient as those in shallow water species.},
doi = {10.1021/es9902322},
journal = {Environmental Science and Technology},
number = 16,
volume = 33,
place = {United States},
year = {1999},
month = {8}
}