Four RFLPs of the human insulin receptor gene: PstI, KpnI, RsaI (2 RFLPs)

Journal Article · Thu Aug 25 00:00:00 EDT 1988 · Nucleic Acids Research; (UK)

DOI:https://doi.org/10.1093/nar/16.16.8204· OSTI ID:6871872

Cox, N J; Spielman, R S; Taub, R ^[1]; Kahn, C R; Muller-Wieland, D; Kriauciunas, K M ^[2]

---

1. Univ. of Pennsylvania School of Medicine, Philadelphia (USA)
2. Harvard Medical School, Boston, MA (USA)

Fragments were isolated from subclones containing the insulin receptor cDNA described. Probe 1 as obtained from an SP64 subclone containing the 1011bp EcoRI fragment from the 5{prime} region of the U11rich cDNA. Probe 1 was a 677bp XhoI/EcoRI fragment from the {alpha} subunit region of the IR cDNA corresponding to nucleotides 334 to 1011, the putative ligand binding domain. Probe 2 was obtained from an SP64 subclone containing the 4190bp EcoRI fragment from the 3{prime} end of the U11rich cDNA. Probe 2 was a 1599 bp PstI fragment from the {beta} subunit region of the insulin receptor cDNA corresponding to nucleotides 2746 to 4345, encoding the tyrosine kinase domain. Segregation in at least one family was observed for the PstI, KpnI, and RsaI ({beta}) polymorphisms.

• OSTI ID: 6871872
• Journal Information: Nucleic Acids Research; (UK), Vol. 16:16; ISSN 0305-1048
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
INSULIN
RECEPTORS
RFLPS
DNA HYBRIDIZATION
DNA SEQUENCING
GENETIC MAPPING
HETEROCHROMOSOMES
HUMAN POPULATIONS
RECOMBINANT DNA
CHROMOSOMES
DNA
HORMONES
HYBRIDIZATION
MAPPING
MEMBRANE PROTEINS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PEPTIDE HORMONES
POPULATIONS
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
550200* - Biochemistry