Effects of class I heparin binding growth factor and fibronectin on platelet adhesion and aggregation

Journal Article · Tue May 01 00:00:00 EDT 1990 · Journal of Vascular Surgery; (USA)

DOI:https://doi.org/10.1016/0741-5214(90)90212-S· OSTI ID:6871186

Greisler, H P; Klosak, J J; Steinam, S J; Lam, T M; Burgess, W H; Kim, D U ^[1]

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1. Loyola Univ. Medical Center Department of Surgery, Maywood, IL (USA)

Fibronectin and heparin binding growth factor-type 1 have been affixed to vascular graft surfaces to enhance the attachment and the proliferation of transplanted endothelial cells, respectively. The current study examines the effect of fibronectin and heparin binding growth factor-type 1 on platelet adhesion and activation in vivo and on platelet aggregation in vitro. Expanded polytetrafluoroethylene prostheses (5 cm x 4 mm internal diameter) were treated either with fibronectin (n = 9), fibronectin/heparin/heparin binding growth factor-type 1/heparin (n = 12), or neither (n = 13) and were interposed into canine aortoiliac systems bilaterally. Autogenous radiolabeled (Indium 111 oxine, 650 microCi) platelets were injected intravenously before reestablishment of circulation. Perfusion was maintained for 30 minutes, and prostheses were removed with segments of native aorta and distal iliac arteries bilaterally. Specimens were examined for thrombus-free surface area, by gamma well counting for adherent radiolabeled platelets, and by light microscopy and transmission and scanning electron microscopic techniques. Results showed that both the fibronectin and fibronectin/heparin/heparin binding growth factor-type 1/heparin pretreated prostheses contained significantly greater numbers of platelets and adherent radioactivity than did control graft segments when normalized to their ipsilateral iliac arteries. Fibronectin/heparin/heparin binding growth factor-type 1/heparin pretreated prostheses contained 27 +/- 16 times more radioactivity per square millimeter than ipsilateral iliac arteries, fibronectin pretreated prostheses had 13 +/- 8 times more radioactivity per square millimeter than ipsilateral iliac arteries, and untreated expanded polytetrafluoroethylene had 4 +/- 3 times more radioactivity per square millimeter than ipsilateral iliac arteries.

• OSTI ID: 6871186
• Journal Information: Journal of Vascular Surgery; (USA), Vol. 11:5; ISSN 0741-5214
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BLOOD PLATELETS
ADHESION
BLOOD VESSELS
GRAFTS
GROWTH FACTORS
BIOCHEMICAL REACTION KINETICS
AORTA
BLOOD COAGULATION
CELL PROLIFERATION
DOGS
ENDOTHELIUM
HEPARIN
IN VITRO
IN VIVO
INDIUM 111
OXINE
PERFUSED TISSUES
SCANNING ELECTRON MICROSCOPY
TRACER TECHNIQUES
TRANSMISSION ELECTRON MICROSCOPY
AMINES
ANIMAL TISSUES
ANIMALS
ANTICOAGULANTS
AROMATICS
ARTERIES
AZAARENES
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOHYDRATES
CARDIOVASCULAR SYSTEM
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ELECTRON MICROSCOPY
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MATERIALS
MICROSCOPY
MINUTES LIVING RADIOISOTOPES
MITOGENS
MUCOPOLYSACCHARIDES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
POLYSACCHARIDES
PROTEINS
PYRIDINES
QUINOLINES
RADIOISOTOPES
REACTION KINETICS
SACCHARIDES
TISSUES
TRANSPLANTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques