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Title: In vivo modulation with anti-interleukin-1 (IL-1) receptor (p80) antibody 35F5 of the response to IL-1. The relationship of radioprotection, colony-stimulating factor, and IL-6

Abstract

Interleukin-1 (IL-1) is radioprotective and induces both circulating colony-stimulating factor(s) (CSF) and IL-6 in mice. We evaluated the relationship among these three responses to IL-1 using anti-IL-1 receptor antibody 35F5. This antibody in vitro blocks responses of T cells and fibroblasts, but not of B cells or myeloid cell lines, to IL-1. Administration of 35F5 alone before irradiation reduced the number of surviving mice compared with those not treated with 35F5, demonstrating that endogenous IL-1 participates in the natural resistance to radiation. Thirty micrograms of 35F5 per mouse also reduced by 92% the survival of irradiated mice pretreated with 0.3 micrograms of IL-1. Similarly, 30 micrograms of 35F5 reduced by 96% to 98% the induction of IL-6 by IL-1. In contrast, 30 micrograms of 35F5 resulted in only moderate reduction of circulating CSF. Consequently, the level of circulating CSF after 35F5 treatment was still equivalent to levels of CSF that were induced by doses of IL-1 in the radioprotective range. Because treatment with 35F5 antibody resulted in the blocking of IL-1-reduced radioprotection, the above results suggest that circulating CSF, by itself, may not be sufficient for radioprotection. This conclusion supports our previous results which showed that granulocyte-macrophage CSF (GM-CSF) andmore » G-CSF were radioprotective only when administered with suboptimal doses of IL-1.« less

Authors:
; ; ; ; ; ;  [1]
  1. Armed Forces Radiobiology Research Institute, Bethesda, MD (USA)
Publication Date:
OSTI Identifier:
6869030
Resource Type:
Journal Article
Journal Name:
Blood (Journal of Hematology); (USA)
Additional Journal Information:
Journal Volume: 76:1; Journal ID: ISSN 0006-4971
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; LYMPHOKINES; RADIOSENSITIVITY EFFECTS; BONE MARROW; CELL CULTURES; DOSE-RESPONSE RELATIONSHIPS; FIBROBLASTS; GROWTH FACTORS; LYMPHOCYTES; MICE; MONOCLONAL ANTIBODIES; RADIATION PROTECTION; RADIOPROTECTIVE SUBSTANCES; RECEPTORS; ANIMAL CELLS; ANIMAL TISSUES; ANIMALS; ANTIBODIES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY; BODY FLUIDS; CONNECTIVE TISSUE CELLS; DRUGS; HEMATOPOIETIC SYSTEM; LEUKOCYTES; MAMMALS; MATERIALS; MEMBRANE PROTEINS; MITOGENS; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RODENTS; SOMATIC CELLS; TISSUES; VERTEBRATES; 560152* - Radiation Effects on Animals- Animals

Citation Formats

Neta, R, Vogel, S N, Plocinski, J M, Tare, N S, Benjamin, W, Chizzonite, R, and Pilcher, M. In vivo modulation with anti-interleukin-1 (IL-1) receptor (p80) antibody 35F5 of the response to IL-1. The relationship of radioprotection, colony-stimulating factor, and IL-6. United States: N. p., 1990. Web.
Neta, R, Vogel, S N, Plocinski, J M, Tare, N S, Benjamin, W, Chizzonite, R, & Pilcher, M. In vivo modulation with anti-interleukin-1 (IL-1) receptor (p80) antibody 35F5 of the response to IL-1. The relationship of radioprotection, colony-stimulating factor, and IL-6. United States.
Neta, R, Vogel, S N, Plocinski, J M, Tare, N S, Benjamin, W, Chizzonite, R, and Pilcher, M. 1990. "In vivo modulation with anti-interleukin-1 (IL-1) receptor (p80) antibody 35F5 of the response to IL-1. The relationship of radioprotection, colony-stimulating factor, and IL-6". United States.
@article{osti_6869030,
title = {In vivo modulation with anti-interleukin-1 (IL-1) receptor (p80) antibody 35F5 of the response to IL-1. The relationship of radioprotection, colony-stimulating factor, and IL-6},
author = {Neta, R and Vogel, S N and Plocinski, J M and Tare, N S and Benjamin, W and Chizzonite, R and Pilcher, M},
abstractNote = {Interleukin-1 (IL-1) is radioprotective and induces both circulating colony-stimulating factor(s) (CSF) and IL-6 in mice. We evaluated the relationship among these three responses to IL-1 using anti-IL-1 receptor antibody 35F5. This antibody in vitro blocks responses of T cells and fibroblasts, but not of B cells or myeloid cell lines, to IL-1. Administration of 35F5 alone before irradiation reduced the number of surviving mice compared with those not treated with 35F5, demonstrating that endogenous IL-1 participates in the natural resistance to radiation. Thirty micrograms of 35F5 per mouse also reduced by 92% the survival of irradiated mice pretreated with 0.3 micrograms of IL-1. Similarly, 30 micrograms of 35F5 reduced by 96% to 98% the induction of IL-6 by IL-1. In contrast, 30 micrograms of 35F5 resulted in only moderate reduction of circulating CSF. Consequently, the level of circulating CSF after 35F5 treatment was still equivalent to levels of CSF that were induced by doses of IL-1 in the radioprotective range. Because treatment with 35F5 antibody resulted in the blocking of IL-1-reduced radioprotection, the above results suggest that circulating CSF, by itself, may not be sufficient for radioprotection. This conclusion supports our previous results which showed that granulocyte-macrophage CSF (GM-CSF) and G-CSF were radioprotective only when administered with suboptimal doses of IL-1.},
doi = {},
url = {https://www.osti.gov/biblio/6869030}, journal = {Blood (Journal of Hematology); (USA)},
issn = {0006-4971},
number = ,
volume = 76:1,
place = {United States},
year = {Sun Jul 01 00:00:00 EDT 1990},
month = {Sun Jul 01 00:00:00 EDT 1990}
}