Isolation and derivatization of plasma taurine for stable isotope analysis by gas chromatography-mass spectrometry
A method for the isolation and derivatization of plasma taurine is described that allows stable isotope determinations of taurine to be made by gas chromatography-mass spectrometry. The isolation procedure can be applied to 0.1 ml of plasma; the recovery of plasma taurine was 70 to 80%. For gc separation, taurine was converted to its dimethylaminomethylene methyl ester derivative which could not be detected by hydrogen flame ionization, but could be monitored readily by NH/sub 3/ chemical ionization mass spectrometry. The derivatization reaction occurred partially on-column and required optimization of injection conditions. Using stable isotope ratiometry multiple ion detection, (M + 2 + H)/sup +//(M + H)/sup +/ ion ratio of natural abundance taurine was determined with a standard deviation of less than +-0.07% of the ratio. The (1,2-/sup 13/C)taurine/taurine mole ratios of standard mixtures could be accurately determined to 0.001. This stable isotope gc-ms method is suitable for studying the plasma kinetics of (1,2-/sup 13/C)taurine in infants who are at risk with respect to taurine depletion.
- Research Organization:
- Argonne National Lab., IL
- DOE Contract Number:
- W-31-109-ENG-38
- OSTI ID:
- 6868727
- Journal Information:
- Anal. Biochem.; (United States), Vol. 107:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
TAURINE
RADIOCHEMICAL ANALYSIS
BIOCHEMICAL REACTION KINETICS
CARBON 13
CHEMICAL COMPOSITION
CHEMICAL PREPARATION
GAS CHROMATOGRAPHY
INFANTS
ISOTOPE RATIO
MASS SPECTROSCOPY
AGE GROUPS
AMINES
ANIMALS
CARBON ISOTOPES
CHEMICAL ANALYSIS
CHILDREN
CHROMATOGRAPHY
EVEN-ODD NUCLEI
ISOTOPES
KINETICS
LIGHT NUCLEI
MAMMALS
MAN
NEONATES
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PRIMATES
QUANTITATIVE CHEMICAL ANALYSIS
REACTION KINETICS
SEPARATION PROCESSES
SPECTROSCOPY
STABLE ISOTOPES
SULFONIC ACIDS
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques