Effect of dietary calcium on bone metabolism in young and aged female rats using a short-term in vivo model
The use of high dietary calcium supplementation in the treatment of patients with osteoporosis is controversial. The present study examined the mechanisms underlying the effects of calcium supplementation by investigating the influence of dietary calcium on bone dynamics in young and aged rats. A 2 x 2 x 2 factorial design was utilized with 0.2% (low) or 1.0% (high) calcium, 2- or 24-m-old female Long-Evans rats that were implanted subcutaneously with demineralized (DB) and mineralized (MB) bone powder. The four groups of rats were fed each of the respective diets for 11 wk and then implanted with one number5 gelatin capsule containing 30 mg of DB and another containing 100 mg of MB powder. The animals were injected intraperitoneally with 0.1 microCi/g body weight with 45Ca 14 h before the end of experiment. The ectopic bone as well as the right femurs were harvested 14 d after the rats were implanted. Marker enzyme activities (alkaline-formation and acid-resorption phosphatase), 45Ca uptake and calcium content were measured in the implants and the distal epiphyses of the right femurs. Bone turnover was higher in the young rats than in the old animals, and high dietary calcium in the young animals increased bone formation, as indicated by alkaline phosphatase activity. Dietary calcium level did not affect ectopic bone formation or resorption in the aged rats. The results indicate that high dietary intake of calcium does not affect bone dynamics in aged female rats but does increase bone formation in young rats.
- Research Organization:
- Univ. of Maryland, College Park (USA)
- OSTI ID:
- 6868118
- Journal Information:
- J. Nutr.; (United States), Vol. 118:10
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ACID PHOSPHATASE
ENZYME ACTIVITY
ALKALINE PHOSPHATASE
CALCIUM
UPTAKE
OSTEOPOROSIS
PATHOGENESIS
AGE DEPENDENCE
BIOLOGICAL MODELS
CALCIUM 45
DIET
METABOLISM
RATS
SKELETON
TRACER TECHNIQUES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METALS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CALCIUM ISOTOPES
DAYS LIVING RADIOISOTOPES
DISEASES
ELEMENTS
ENZYMES
ESTERASES
EVEN-ODD NUCLEI
HYDROLASES
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
METALS
NUCLEI
ORGANS
PHOSPHATASES
RADIOISOTOPES
RODENTS
SKELETAL DISEASES
VERTEBRATES
550501* - Metabolism- Tracer Techniques
550901 - Pathology- Tracer Techniques