Influence of dose rate on the transformation of Syrian hamster embryo cells by fission-spectrum neutrons
Several explanations for this neutron dose-rate effect have been proposed, but further investigation is necessary to determine the mechanisms involved. In all cell transformation studies to date the immortalized, aneuploid 10T1/2 cell-line has been used. These cells may be premalignant; thus their response characteristics and, in particular, the nature of the transformation event, might differ from that in a normal, fibroblast cell. One reason for the present study was to determine whether the low-dose-rate effect of fission neutrons could be demonstrated in normal cells. If so, a normal cell system, which would more closely resemble a normal in vivo system, could be used for mechanistic studies. We chose Syrian hamster embryo (SHE) fibroblasts which are normal, diploid cells with a limited life span in culture. Upon exposure to low doses of ionizing radiation, the fraction of the cells that are transformed can be identified in a standard 8--10 day colony assay by examining their clonal morphology. Transformed cells form colonies with a dense, criss-crossed or piled-up structure. A high percentage of the transformed colonies can be further propagated and will acquire additional neoplastic characteristics; i.e., anchorage independence, immortality, altered proteolytic activity, karyotype alterations, and finally, tumorigenicity.
- Research Organization:
- Argonne National Lab., IL (USA)
- DOE Contract Number:
- W-31109-ENG-38
- OSTI ID:
- 6865648
- Report Number(s):
- CONF-8809137-5; ON: DE89003657; TRN: 89-001128
- Resource Relation:
- Conference: 14. L. H. Gray conference, Oxford, UK, 11 Sep 1988; Other Information: Portions of this document are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
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NEUTRONS
RISK ASSESSMENT
RBE
DOSE-RESPONSE RELATIONSHIPS
A-BOMB SURVIVORS
BIOLOGICAL EFFECTS
CARCINOGENESIS
CELL CULTURES
CELL TRANSFORMATIONS
DOSE RATES
DOSIMETRY
EMBRYONIC CELLS
ENZYME INDUCTION
FISSION NEUTRONS
HEALTH HAZARDS
MICE
RADIOBIOLOGY
ANIMAL CELLS
ANIMALS
BARYONS
BIOLOGY
ELEMENTARY PARTICLES
FERMIONS
GENE REGULATION
HADRONS
HAZARDS
HUMAN POPULATIONS
MAMMALS
NUCLEONS
PATHOGENESIS
POPULATIONS
RODENTS
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
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