Direct electrospray ion current monitoring detection and its use with on-line capillary electrophoresis mass spectrometry
- Pacific Northwest Lab., Richland, WA (United States)
A novel detection scheme for use with the capillary electrophoresis-electrospray ionization interface is presented, based upon the direct measurement of electrospray ion current after expansion into vacuum, that is shown to provide the basis for a simple detector or for simultaneous dual detection with mass spectrometry. The utility of this novel dual-detection scheme is illustrated with capillary electrophoresis Fourier transform ion cyclotron resonance (FTICR) mass spectrometry in which spectra are acquired only when a solute zone elutes from the CE capillary. This acquisition scheme affords significantly improved sensitivity and permits the acquisition of longer time domain signals without sacrificing separation efficiency. Mass resolving power in excess of 10[sup 5] (fwhm) is demonstrated by FTICR for components from a peptide/protein mixture comprised of analytes with molecular masses ranging from 2 to 29 kDa. 24 refs., 3 figs.
- DOE Contract Number:
- AC06-76RL01830
- OSTI ID:
- 6864674
- Journal Information:
- Analytical Chemistry (Washington); (United States), Vol. 67:2; ISSN 0003-2700
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
MASS SPECTROMETERS
USES
CAPILLARIES
ELECTRIC CURRENTS
ELECTROPHORESIS
FOURIER TRANSFORM SPECTROMETERS
IONS
MOLECULAR WEIGHT
MONITORING
PROTEINS
SPRAYED COATINGS
BLOOD VESSELS
BODY
CARDIOVASCULAR SYSTEM
CHARGED PARTICLES
COATINGS
CURRENTS
MEASURING INSTRUMENTS
ORGANIC COMPOUNDS
ORGANS
SPECTROMETERS
440101* - Radiation Instrumentation- General Detectors or Monitors & Radiometric Instruments
440103 - Radiation Instrumentation- Nuclear Spectroscopic Instrumentation
400400 - Electrochemistry