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Title: Detection of hydroxylated nitro aromatic and hydroxylated nitro polycyclic aromatic compounds in an ambient air particulate extract using bioassay-directed fractionation

Abstract

The identification and quantification of hydroxylated nitro aromatic compounds in an ambient air particulate extract are presented here. The isomer substitution observed for these compounds supports the mechanism proposed from smog chamber studies: OH attack on the aromatic compound followed by nitration ortho or para to the OH substitutent. Several hydroxylated nitro polycyclic aromatic compounds have been tentatively identified and quantified in the extract also. Both electron ionization (EI) and negative chemical ionization (NCI) high-resolution gas chromatography/mass spectrometry. (GC/MS) analyses were used to identify these components. Bioassay-directed fractionation through three sequential separations led to detection of the hydroxylated nitro polycyclic compounds in a mutagenic and substantially simplified subfraction of the extract.

Authors:
; ; ; ;
Publication Date:
Research Org.:
Battelle Columbus Division, Columbus, OH (USA)
OSTI Identifier:
6861327
Resource Type:
Journal Article
Journal Name:
Environ. Sci. Technol.; (United States)
Additional Journal Information:
Journal Volume: 22:8
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 54 ENVIRONMENTAL SCIENCES; NITRO COMPOUNDS; FRACTIONATION; POLYCYCLIC AROMATIC HYDROCARBONS; AIR POLLUTION; GAS CHROMATOGRAPHY; MASS SPECTROSCOPY; MUTAGEN SCREENING; PARTICULATES; AROMATICS; CHROMATOGRAPHY; HYDROCARBONS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PARTICLES; POLLUTION; SCREENING; SEPARATION PROCESSES; SPECTROSCOPY; 560300* - Chemicals Metabolism & Toxicology; 500200 - Environment, Atmospheric- Chemicals Monitoring & Transport- (-1989)

Citation Formats

Nishioka, M G, Howard, C C, Contos, D A, Ball, L M, and Lewtas, J. Detection of hydroxylated nitro aromatic and hydroxylated nitro polycyclic aromatic compounds in an ambient air particulate extract using bioassay-directed fractionation. United States: N. p., 1988. Web. doi:10.1021/es00173a007.
Nishioka, M G, Howard, C C, Contos, D A, Ball, L M, & Lewtas, J. Detection of hydroxylated nitro aromatic and hydroxylated nitro polycyclic aromatic compounds in an ambient air particulate extract using bioassay-directed fractionation. United States. doi:10.1021/es00173a007.
Nishioka, M G, Howard, C C, Contos, D A, Ball, L M, and Lewtas, J. Mon . "Detection of hydroxylated nitro aromatic and hydroxylated nitro polycyclic aromatic compounds in an ambient air particulate extract using bioassay-directed fractionation". United States. doi:10.1021/es00173a007.
@article{osti_6861327,
title = {Detection of hydroxylated nitro aromatic and hydroxylated nitro polycyclic aromatic compounds in an ambient air particulate extract using bioassay-directed fractionation},
author = {Nishioka, M G and Howard, C C and Contos, D A and Ball, L M and Lewtas, J},
abstractNote = {The identification and quantification of hydroxylated nitro aromatic compounds in an ambient air particulate extract are presented here. The isomer substitution observed for these compounds supports the mechanism proposed from smog chamber studies: OH attack on the aromatic compound followed by nitration ortho or para to the OH substitutent. Several hydroxylated nitro polycyclic aromatic compounds have been tentatively identified and quantified in the extract also. Both electron ionization (EI) and negative chemical ionization (NCI) high-resolution gas chromatography/mass spectrometry. (GC/MS) analyses were used to identify these components. Bioassay-directed fractionation through three sequential separations led to detection of the hydroxylated nitro polycyclic compounds in a mutagenic and substantially simplified subfraction of the extract.},
doi = {10.1021/es00173a007},
journal = {Environ. Sci. Technol.; (United States)},
number = ,
volume = 22:8,
place = {United States},
year = {1988},
month = {8}
}