Membrane-associated precursor to poliovirus VPg identified by immunoprecipitation with antibodies directed against a synthetic heptapeptide

Journal Article · Mon Feb 01 00:00:00 EST 1982 · Cell; (United States)

DOI:https://doi.org/10.1016/0092-8674(82)90358-0· OSTI ID:6857527

Semelr, B L ^[1]; Anderson, C W; Hanecak, R; Dorner, L F; Wimmer, E

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1. State Univ. of New York, Stony Brook

A synthetic heptapeptide corresponding to the C-terminal sequence of the poliovirus genome protein (VPg) has been linked to bovine serum albumin and used to raise antibodies in rabbits. These antibodies precipitate not only VPg but also at least two more virus-specific polypeptides. The smaller polypeptide, denoted P3-9 (12,000 daltons), has been mapped by Edman degradation and by fragmentation with cyanogen bromide and determined to be the N-terminal cleavage product of polypeptide P3-1b, a precursor to the RNA polymerase. P3-9 contains the sequence of the basic protein VPg (22 amino acids) at its C terminus. As predicted by the known RNA sequence of poliovirus, P3-9 also contains a hydrophobic region of 22 amino acids preceding VPg, an observation suggesting that P3-9 may be membrane-associated. This was confirmed by fractionation of infected cells in the presence or absence of detergent. We speculate that P3-9 may be the donor of VPg to RNA chains in the membrane-bound RNA replication complex.

• OSTI ID: 6857527
• Journal Information: Cell; (United States), Vol. 28
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
POLIO VIRUS
BIOCHEMISTRY
POLYPEPTIDES
PRECIPITATION
ALBUMINS
AMINO ACIDS
ANTIBODY FORMATION
AUTORADIOGRAPHY
BROMIDES
CELL MEMBRANES
CYANOGEN
ELECTROPHORESIS
HELA CELLS
IODINE 125
LABELLED COMPOUNDS
RABBITS
TRITIUM COMPOUNDS
TYROSINE
ANIMALS
BETA DECAY RADIOISOTOPES
BROMINE COMPOUNDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMISTRY
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
HALIDES
HALOGEN COMPOUNDS
HYDROXY ACIDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
MAMMALS
MEMBRANES
MICROORGANISMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PEPTIDES
PROTEINS
RADIOISOTOPES
SEPARATION PROCESSES
VERTEBRATES
VIRUSES
550201* - Biochemistry- Tracer Techniques
550701 - Microbiology- Tracer Techniques