Inducible cadmium binding complexes of cabbage and tobacco
Cadmium complexes with apparent molecular weights of 10,000 were observed in aqueous extracts of Cd-treated cabbage (Brassica capitata L., cv. red danish) and tobacco (hybrid of Nicotiana glauca and N. langsdorffii) plants. The amount of complex (as Cd) recovered was found to be dependent on the concentration of the metal in the growth medium and the total time of exposure of plants to the metal. Induction of the complex at moderate levels of /sup 112/Cd exposure was monitored after labeling the complex with /sup 109/Cd in vitro. The constitutive nature of the ligand of the complex in cabbage and tobacco leaves was suggested when control plant extracts were exposed to /sup 109/Cd. Such extracts contained /sup 109/Cd, which eluted froom Sephadex G-50 in the region of Cd complex. Simultaneous labeling with /sup 112/Cd and /sup 35/S or /sup 32/P indicated that the complex contained sulfur but probably not phosphorus. The amount of /sup 35/S which eluted coincident with /sup 112/Cd complex increased during complex induction. No evidence was found for the presence of 10,000 molecular weight Cd complex in stem exudates (vascular sap) of Cd-treated plants. The results obtained are consistent with the presence in these tissues of a ligand which is both inducible and consitutive and binds Cd in mercaptide bonds. All of these properties and oters reported earlier, are characteristic of Cd-metallothionein formed in animals.
- Research Organization:
- Brookhaven National Lab. (BNL), Upton, NY (United States)
- OSTI ID:
- 6857427
- Journal Information:
- Plant Physiol.; (United States), Vol. 69
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CADMIUM 112
BIOLOGICAL EFFECTS
CADMIUM COMPLEXES
CHEMICAL ACTIVATION
PHOSPHORUS 32
SULFUR 35
BRASSICA
CADMIUM 109
IN VITRO
LIGANDS
LIQUID COLUMN CHROMATOGRAPHY
METALLOTHIONEIN
MOLECULAR WEIGHT
NICOTIANA
PHYSIOLOGY
TRACER TECHNIQUES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CADMIUM ISOTOPES
CHROMATOGRAPHY
COMPLEXES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EVEN-EVEN NUCLEI
EVEN-ODD NUCLEI
FOOD
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
METALLOPROTEINS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PLANTS
PROTEINS
RADIOISOTOPES
SEPARATION PROCESSES
STABLE ISOTOPES
SULFUR ISOTOPES
VEGETABLES
YEARS LIVING RADIOISOTOPES
550201* - Biochemistry- Tracer Techniques
551001 - Physiological Systems- Tracer Techniques