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Lumen formation by epithelial cell lines in response to collagen overlay: a morphogenetic model in culture
Abstract
Two cells lines--Madin-Darby canine kidney (MDCK) and normal murine mammary gland (NMuMG)--growing as monolayers on collagen gels were overlaid with another collagen gel. The cells responded to the overlay by undergoing reorganization resulting in the creation of lumina. MDCK cells formed lumina that coalesced to form large cavities comparable in size with a tubule. NMuMG cells formed clusters surrounding small lumina, which appeared similar to acini of glandular tissue. The characteristic arrangements, described here by light and electron microscopy, resembled the morphology of the tissues of cell line origin. MDCK cells, grown in the presence of serum, formed lumina whether or not serum was removed at the time of overlay, whereas NMuMG cells required either a nondialyzable component of serum or hormonal supplements in serum-free defined media. Lumen formation was delayed by MDCK cells in the presence of the glutamine analog 6-diazo-5-oxo-L-norleucine, but this compound did not affect NMuMG lumen formation. In both cell lines, lumen formation was unaffected by the absence of sulfate, the presence of an inhibitor of sulfate glycosaminoglycan synthesis, or an inhibitor of collagen synthesis. DNA synthesis accompanied lumen formation but was not required.
- Authors:
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- OSTI Identifier:
- 6857247
- DOE Contract Number:
- AC03-76SF00098
- Resource Type:
- Journal Article
- Journal Name:
- Proc. Natl. Acad. Sci. U.S.A.; (United States)
- Additional Journal Information:
- Journal Volume: 79:15
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; ANIMAL CELLS; MORPHOLOGICAL CHANGES; COLLAGEN; BIOLOGICAL EFFECTS; BIOSYNTHESIS; CELL CULTURES; DNA; DOGS; INHIBITION; KIDNEYS; MAMMARY GLANDS; MICE; SULFATES; ANIMALS; BODY; GLANDS; MAMMALS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANS; OXYGEN COMPOUNDS; PROTEINS; RODENTS; SCLEROPROTEINS; SULFUR COMPOUNDS; SYNTHESIS; VERTEBRATES; 550300* - Cytology
Citation Formats
Hall, H G, Farson, D A, and Bissel, M J. Lumen formation by epithelial cell lines in response to collagen overlay: a morphogenetic model in culture. United States: N. p., 1982.
Web. doi:10.1073/pnas.79.15.4672.
Hall, H G, Farson, D A, & Bissel, M J. Lumen formation by epithelial cell lines in response to collagen overlay: a morphogenetic model in culture. United States. https://doi.org/10.1073/pnas.79.15.4672
Hall, H G, Farson, D A, and Bissel, M J. 1982.
"Lumen formation by epithelial cell lines in response to collagen overlay: a morphogenetic model in culture". United States. https://doi.org/10.1073/pnas.79.15.4672.
@article{osti_6857247,
title = {Lumen formation by epithelial cell lines in response to collagen overlay: a morphogenetic model in culture},
author = {Hall, H G and Farson, D A and Bissel, M J},
abstractNote = {Two cells lines--Madin-Darby canine kidney (MDCK) and normal murine mammary gland (NMuMG)--growing as monolayers on collagen gels were overlaid with another collagen gel. The cells responded to the overlay by undergoing reorganization resulting in the creation of lumina. MDCK cells formed lumina that coalesced to form large cavities comparable in size with a tubule. NMuMG cells formed clusters surrounding small lumina, which appeared similar to acini of glandular tissue. The characteristic arrangements, described here by light and electron microscopy, resembled the morphology of the tissues of cell line origin. MDCK cells, grown in the presence of serum, formed lumina whether or not serum was removed at the time of overlay, whereas NMuMG cells required either a nondialyzable component of serum or hormonal supplements in serum-free defined media. Lumen formation was delayed by MDCK cells in the presence of the glutamine analog 6-diazo-5-oxo-L-norleucine, but this compound did not affect NMuMG lumen formation. In both cell lines, lumen formation was unaffected by the absence of sulfate, the presence of an inhibitor of sulfate glycosaminoglycan synthesis, or an inhibitor of collagen synthesis. DNA synthesis accompanied lumen formation but was not required.},
doi = {10.1073/pnas.79.15.4672},
url = {https://www.osti.gov/biblio/6857247},
journal = {Proc. Natl. Acad. Sci. U.S.A.; (United States)},
number = ,
volume = 79:15,
place = {United States},
year = {Sun Aug 01 00:00:00 EDT 1982},
month = {Sun Aug 01 00:00:00 EDT 1982}
}
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