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Title: Fractionation, characterization, and subcellular localization of colony-stimulating activities released by the human monocyte-like cell line, GCT

Abstract

GCT, a human monocyte-like cell line, has been shown to release biochemically distinct colony-stimulating activities (CSAs) for mouse and human marrows. These appear to be periodate-sensitive proteins with critical disulfide bonds. One, of molecular weight 145,000 daltons, stimulates mouse macrophagic colony growth and is related to a 30,000-dalton molecule that also stimulates mouse growth. A 30,000-dalton CSA for human marrow can be separated from the 30,000-dalton mouse CSA. The 30,000-dalton CSAs stimulate neutrophil, neutrophil-monocyte, and eosinophil colony growth in human marrow but only neutrophil and neutrophil-monocyte colonies in the mouse. Subcellular fractionation of GCT cells indicates that there are pools of preformed CSAs primarily associated with the cell cytosol that have similar apparent molecular weights to their secreted counterparts.

Authors:
; ; ; ;
Publication Date:
Research Org.:
Univ. of Rochester School of Medicine, NY
OSTI Identifier:
6856446
Alternate Identifier(s):
OSTI ID: 6856446
Resource Type:
Journal Article
Resource Relation:
Journal Name: Blood; (United States); Journal Volume: 56:4
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; COLONY FORMATION; STIMULATION; MONOCYTES; MOLECULAR STRUCTURE; PROTEINS; BIOSYNTHESIS; FRACTIONATION; CELL CULTURES; CYCLOHEXIMIDE; CYTOPLASM; MAN; MICE; ANIMALS; ANTIBIOTICS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CELL CONSTITUENTS; DRUGS; FUNGICIDES; LEUKOCYTES; MAMMALS; MATERIALS; ORGANIC COMPOUNDS; PESTICIDES; PRIMATES; RODENTS; SEPARATION PROCESSES; SYNTHESIS; VERTEBRATES 550200* -- Biochemistry

Citation Formats

DiPersio, J.F., Brennan, J.K., Lichtman, M.A., Abboud, C.N., and Kirkpatrick, F.H. Fractionation, characterization, and subcellular localization of colony-stimulating activities released by the human monocyte-like cell line, GCT. United States: N. p., 1980. Web.
DiPersio, J.F., Brennan, J.K., Lichtman, M.A., Abboud, C.N., & Kirkpatrick, F.H. Fractionation, characterization, and subcellular localization of colony-stimulating activities released by the human monocyte-like cell line, GCT. United States.
DiPersio, J.F., Brennan, J.K., Lichtman, M.A., Abboud, C.N., and Kirkpatrick, F.H. Wed . "Fractionation, characterization, and subcellular localization of colony-stimulating activities released by the human monocyte-like cell line, GCT". United States.
@article{osti_6856446,
title = {Fractionation, characterization, and subcellular localization of colony-stimulating activities released by the human monocyte-like cell line, GCT},
author = {DiPersio, J.F. and Brennan, J.K. and Lichtman, M.A. and Abboud, C.N. and Kirkpatrick, F.H.},
abstractNote = {GCT, a human monocyte-like cell line, has been shown to release biochemically distinct colony-stimulating activities (CSAs) for mouse and human marrows. These appear to be periodate-sensitive proteins with critical disulfide bonds. One, of molecular weight 145,000 daltons, stimulates mouse macrophagic colony growth and is related to a 30,000-dalton molecule that also stimulates mouse growth. A 30,000-dalton CSA for human marrow can be separated from the 30,000-dalton mouse CSA. The 30,000-dalton CSAs stimulate neutrophil, neutrophil-monocyte, and eosinophil colony growth in human marrow but only neutrophil and neutrophil-monocyte colonies in the mouse. Subcellular fractionation of GCT cells indicates that there are pools of preformed CSAs primarily associated with the cell cytosol that have similar apparent molecular weights to their secreted counterparts.},
doi = {},
journal = {Blood; (United States)},
number = ,
volume = 56:4,
place = {United States},
year = {Wed Oct 01 00:00:00 EDT 1980},
month = {Wed Oct 01 00:00:00 EDT 1980}
}