Therapeutic administration of recombinant human granulocyte colony-stimulating factor accelerates hemopoietic regeneration and enhances survival in a murine model of radiation-induced myelosuppression
- Armed Forces Radiobiology Research Institute, Bethesda, MD (USA)
The primary cause of death after radiation exposure is infection resulting from myelosuppression. Because granulocytes play a critical role in host defense against infection and because granulocyte proliferation and differentiation are enhanced by granulocyte colony-stimulating factor (G-CSF), this agent was evaluated for the ability to accelerate hemopoietic regeneration and to enhance survival in irradiated mice. C3H/HeN mice were irradiated and G-CSF (2.5 micrograms/day, s.c.) or saline was administered on days 3-12, 1-12 or 0-12 post-irradiation. Bone marrow, splenic and peripheral blood cellularity, and bone marrow and splenic granulocyte-macrophage progenitor cell recoveries were evaluated in mice exposed to 6.5 Gy. Mice exposed to 8 Gy were evaluated for multipotent hemopoietic stem cell recovery (using endogenous spleen colony-forming units) and enhanced survival. Results demonstrated that therapeutic G-CSF (1) accelerates hemopoietic regeneration after radiation-induced myelosuppression, (2) enhances survival after potentially lethal irradiation and (3) is most effective when initiated 1 h following exposure.
- OSTI ID:
- 6853390
- Journal Information:
- International Journal of Cell Cloning; (USA), Vol. 8:2; ISSN 0737-1454
- Country of Publication:
- United States
- Language:
- English
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Survival enhancement and hemopoietic regeneration following radiation exposure: Therapeutic approach using glucan and granulocyte colony-stimulating factor
Survival enhancement and hemopoietic regeneration following radiation exposure: therapeutic approach using glucan and granulocyte colony-stimulating factor
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GROWTH FACTORS
RADIOSENSITIVITY EFFECTS
LEUKOCYTES
CELL PROLIFERATION
ANEMIAS
MICE
RADIATION INJURIES
SURVIVAL TIME
WHOLE-BODY IRRADIATION
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
DISEASES
EXTERNAL IRRADIATION
HEMIC DISEASES
INJURIES
IRRADIATION
MAMMALS
MATERIALS
MITOGENS
ORGANIC COMPOUNDS
PROTEINS
RADIATION EFFECTS
RODENTS
SYMPTOMS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals