Disposition of naphthalene and its metabolites in the brain of rainbow trout (Salmo gairdneri)
Rainbow trout (Salmo gairdneri) were exposed to orally administered (/sup 3/H)naphthalene. Another group received naphthyl glucuronic acid and naphthyl sulfate via iv injection. Brain, liver, and blood were assayed for the parent compound and/or total metabolites. Individual naphthalene derivatives were determined by high-performance liquid chromatography (hplc) using either radiometric or on-line fluorimetric detection systems. Naphthalene concentrations in brain (8.2 pmol/mg dry wt at 16 hr after feeding) approximated those found at the same time in liver (7.4 pmol/mg dry wt). A nonconjugated naphthalene derivative, 1,2-dihydro-1,2-dihydroxynaphthalene, also accumulated in brain (0.041 pmol/mg dry wt after 16 hr), although to a lesser degree than in liver (0.10 pmol/mg dry wt after 16 hr). Conjugated naphthalene derivatives, 1-naphthyl sulfate and 1-naphthyl glucuronic acid, although present in liver and blood, were largely excluded from the brain. Low naphthalene hydroxylase activity (<2.0 pmol product formed/mg protein/min) indicated that the trout brain has a minimal ability to oxidize aromatic hydrocarbons. These findings suggest that the brain of adult trout is substantially different from other tissues (e.g., liver and blood) with respect to the disposition of naphthalene and its metabolites.
- Research Organization:
- National Marine Fisheries Service, Seattle, WA
- OSTI ID:
- 6852015
- Journal Information:
- Environ. Res.; (United States), Vol. 23:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
NAPHTHALENE
TISSUE DISTRIBUTION
TROUT
CONTAMINATION
BLOOD
BRAIN
EXPERIMENTAL DATA
GLUCURONIC ACID
INTRAVENOUS INJECTION
LIQUID COLUMN CHROMATOGRAPHY
LIVER
METABOLITES
ORAL ADMINISTRATION
QUANTITATIVE CHEMICAL ANALYSIS
RETENTION
TIME DEPENDENCE
TRITIUM COMPOUNDS
ALDEHYDES
ANIMALS
AQUATIC ORGANISMS
AROMATICS
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
CHEMICAL ANALYSIS
CHROMATOGRAPHY
CONDENSED AROMATICS
DATA
DIGESTIVE SYSTEM
DISTRIBUTION
FISHES
GLANDS
HYDROCARBONS
HYDROXY ACIDS
INFORMATION
INJECTION
INTAKE
LABELLED COMPOUNDS
MATERIALS
NERVOUS SYSTEM
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
SEPARATION PROCESSES
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
550501 - Metabolism- Tracer Techniques