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Title: RNA polymerase III transcription in higher plants: (Annual) performance report, May 1, 1986 through December 20, 1986

Abstract

From wheat germ, we have isolated and partially purified the RNA polymerase III. We have shown it to be functional for tRNA synthesis in combination with yeast transcription factors tau (TFIIIC) and B. In addition, by the test of specific DNA binding, we have isolated a putative wheat TFIIIC. Several critical tests have been made, using human (HeLa cell) RNA polymerase III and its factors as a model system, to see whether complementation of an incomplete yeast Pol III system with factors of heterologous origin is likely to be successful. 5 refs.

Authors:
Publication Date:
Research Org.:
Washington Univ., Seattle (USA). Dept. of Genetics
OSTI Identifier:
6847174
Alternate Identifier(s):
OSTI ID: 6847174; Legacy ID: DE87005548
Report Number(s):
DOE/ER/13517-1
ON: DE87005548
DOE Contract Number:
FG06-86ER13517
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; NUCLEOPROTEINS; FRACTIONATION; RNA POLYMERASES; TRANSFER RNA; WHEAT; CEREALS; ENZYMES; GRASS; NUCLEIC ACIDS; NUCLEOTIDYLTRANSFERASES; ORGANIC COMPOUNDS; PHOSPHORUS-GROUP TRANSFERASES; PLANTS; POLYMERASES; PROTEINS; RNA; SEPARATION PROCESSES; TRANSFERASES 550200* -- Biochemistry

Citation Formats

Hall, B.D.. RNA polymerase III transcription in higher plants: (Annual) performance report, May 1, 1986 through December 20, 1986. United States: N. p., 1986. Web.
Hall, B.D.. RNA polymerase III transcription in higher plants: (Annual) performance report, May 1, 1986 through December 20, 1986. United States.
Hall, B.D.. Wed . "RNA polymerase III transcription in higher plants: (Annual) performance report, May 1, 1986 through December 20, 1986". United States. doi:.
@article{osti_6847174,
title = {RNA polymerase III transcription in higher plants: (Annual) performance report, May 1, 1986 through December 20, 1986},
author = {Hall, B.D.},
abstractNote = {From wheat germ, we have isolated and partially purified the RNA polymerase III. We have shown it to be functional for tRNA synthesis in combination with yeast transcription factors tau (TFIIIC) and B. In addition, by the test of specific DNA binding, we have isolated a putative wheat TFIIIC. Several critical tests have been made, using human (HeLa cell) RNA polymerase III and its factors as a model system, to see whether complementation of an incomplete yeast Pol III system with factors of heterologous origin is likely to be successful. 5 refs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed Jan 01 00:00:00 EST 1986},
month = {Wed Jan 01 00:00:00 EST 1986}
}

Technical Report:
Other availability
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