Pertussis toxin treatment does not block inhibition by atrial natriuretic factor of aldosterone secretion in cultured bovine zona glomerulosa cells
The authors have previously reported that atrial natriuretic factor (ANF) potently inhibits PGE or forskolin-stimulation aldosterone secretion in bovine zona glomerulosa (ZG) by acting through specific high affinity receptors. In order to evaluate the functional role of the regulatory protein N/sub i/ and the inhibition of adenylate cyclase activity (AC) in ZG, the authors have studied the effect of treatment with PT on inhibition by ANF of aldosterone production. Primary cultures of ZG were treated for 18 hours in serum-free F12 medium with (0-100 ng/ml PT). No effect of PT pretreatment was observed either on basal, PGE-stimulated or ANF-inhibited levels of steroidogenesis. When membranes prepared from control ZG were ADP-ribosylated with (/sup 32/P) NAD in the presence of PT, two toxin-specific bands with 39 Kd and 41 Kd were documented on SDS gel. Cell pretreatment with as low as 1 ng/ml drastically reduced further labelling of these two bands while higher doses completely abolished them. Since PT treatment covalently modifies completely the toxin substrate without altering ANF inhibition of adrenal steroidogenesis, the authors conclude that N/sub i/ is not involved in the mode of action of ANF on aldosterone production.
- Research Organization:
- Institut de Recherches Cliniques de Montreal, Quebec
- OSTI ID:
- 6846332
- Report Number(s):
- CONF-8604222-; TRN: 87-008625
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
Similar Records
Atrial natriuretic factor: radioimmunoassay and effects on adrenal and pituitary glands
Role of calcium in effects of atrial natriuretic peptide on aldosterone production in adrenal glomerulosa cells
Related Subjects
ALDOSTERONE
SECRETION
CYCLASES
ENZYME ACTIVITY
PROTEINS
BIOLOGICAL EFFECTS
TOXINS
BIOCHEMICAL REACTION KINETICS
CATTLE
CELL CULTURES
CELL MEMBRANES
INHIBITION
NAD
PHOSPHORUS 32
TRACER TECHNIQUES
VALENCE
ADRENAL HORMONES
ALDEHYDES
ANIMALS
ANTIGENS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CELL CONSTITUENTS
COENZYMES
CORTICOSTEROIDS
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ENZYMES
HORMONES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
KINETICS
LIGHT NUCLEI
LYASES
MAMMALS
MATERIALS
MEMBRANES
MINERALOCORTICOIDS
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PREGNANES
RADIOISOTOPES
REACTION KINETICS
RUMINANTS
STEROID HORMONES
STEROIDS
TOXIC MATERIALS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques