A real-time kinetic study of luciferase inactivation by pulsed ionizing radiation

Journal Article · Tue Jun 01 00:00:00 EDT 1982 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575729· OSTI ID:6842470

Bell, D H; Gould, J M; Patterson, L K

The real-time kinetics of radiation-induced inactivation of the luminescent firefly luciferase-luciferin system were investigated. A single, microsecond pulse from a Van de Graaff accelerator delivered to the system is sufficient to decrease the luminescence by over 60%. This decrease exhibits exponential behavior and has a half-time of 46 +/- 6 msec. In both steady-state and pulsed studies, the dose dependence of the inactivation is independent of the dose rate. Likewise, the decay kinetics are independent of the dose per pulse. These studies suggest that the enzyme is altered in a way that inteferes with the initial steps of catalysis without affecting the subsequent steps which lead to light emission.

• Research Organization: Univ. of Notre Dame, IN
• OSTI ID: 6842470
• Journal Information: Radiat. Res.; (United States), Vol. 90:3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
LUCIFERASE
BIOLOGICAL RADIATION EFFECTS
INHIBITION
BIOCHEMICAL REACTION KINETICS
COBALT 60
DOSE RATES
DOSE-RESPONSE RELATIONSHIPS
PHOTOLUMINESCENCE
PULSES
RADIOINDUCTION
VAN DE GRAAFF ACCELERATORS
ACCELERATORS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
COBALT ISOTOPES
ELECTROSTATIC ACCELERATORS
ENZYMES
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
KINETICS
LUMINESCENCE
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
OXIDOREDUCTASES
RADIATION EFFECTS
RADIOISOTOPES
REACTION KINETICS
YEARS LIVING RADIOISOTOPES
560111* - Radiation Effects on Biochemicals- In Vitro- (-1987)
550200 - Biochemistry