Biological responsiveness to the phorbol esters and specific binding of (/sup 3/H)phorbol 12,13-dibutyrate in the nematode Caenorhabditis elegans, a manipulable genetic system
Because of its suitability for genetic studies, the nematode Caenorhabditis elegans was examined for its responsiveness to the phorbol esters. Phorbol 12-myristate 13-acetate had three effects. It inhibited the increase in animal size during growth; it decreased the yield of progeny; and it caused uncoordinated movement of the adult. The effects on nematode size, progeny yield, and movement were quantitated. Concentrations of phorbol 12-myristate 13-acetate yielding half-maximal responses were 440, 460, and 170 nM, respectively. As was expected from the biological responsiveness of the nematodes, specific, saturable binding of phorbol ester to nematode extracts was found. (/sup 3/H)phorbol 12,13-dibutyrate bound with a dissociation constant of 26.8 +/- 3.9 nM. At saturation, 5.7 +/- 1.4 pmole/mg protein was bound.
- Research Organization:
- Department of Neuropathology, Harvard Medical School, Boston
- OSTI ID:
- 6841422
- Journal Information:
- Teratog., Carcinog., Mutagen.; (United States), Vol. 2:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
NEMATODES
SENSITIVITY
PHORBOL ESTERS
TOXICITY
TRITIUM COMPOUNDS
TRACER TECHNIQUES
ANIMAL GROWTH
BEHAVIOR
BIOCHEMICAL REACTION KINETICS
DOSE-RESPONSE RELATIONSHIPS
GENETICS
PROGENY
ASCHELMINTHES
BIOLOGY
CARCINOGENS
ESTERS
GROWTH
HELMINTHS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
ORGANIC COMPOUNDS
REACTION KINETICS
560304* - Chemicals Metabolism & Toxicology- Invertebrates- (-1987)
550201 - Biochemistry- Tracer Techniques