Transposable element-mediated enhancement of gene expression in saccharomyces cerevisiae involves sequence-specific binding of a trans-acting factor

Journal Article · Wed Jun 01 00:00:00 EDT 1988 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.8.6.2572· OSTI ID:6838798

Goel, A; Pearlman, R E

In the authors' studies on the regulation of adjacent-gene expression by Ty sequences, they demonstrated that a single-base-pair change (T-A---C-G) in the epsilon sequence of Ty917-derived elements is primarily responsible for enhancement of BETA-galactosidase expression from lacZ fusion plasmids. Using an electrophoretic gel mobility mobility assay, they showed that the same base pair transition is required for binding a trans-acting factor, TyBF, from crude cell extracts in vitro. The authors identified the site of TyBF binding and determined the guanine nucleotide contact sites required for TyBF interaction. They propose that TyBF binding to cis-acting Ty2 sequences activates adjacent-gene transcription.

• Research Organization: Dept. of Biology, York Univ., 4700 Keele Street, Toronto, Ontario (CA)
• OSTI ID: 6838798
• Journal Information: Mol. Cell. Biol.; (United States), Vol. 8:6
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GALACTOSIDASE
GENE REGULATION
TRANSPOSONS
BIOLOGICAL FUNCTIONS
MOLECULAR BIOLOGY
BIOLOGICAL PATHWAYS
DNA BASE TRANSITIONS
DNA SEQUENCING
GENE AMPLIFICATION
GENE REPRESSORS
HYBRIDIZATION
IN VITRO
NUCLEOPROTEINS
PHENOTYPE
PLASMIDS
SACCHAROMYCES CEREVISIAE
TRANSCRIPTION
CELL CONSTITUENTS
ENZYMES
FUNCTIONS
FUNGI
GLYCOSYL HYDROLASES
HYDROLASES
MICROORGANISMS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PLANTS
PROTEINS
SACCHAROMYCES
STRUCTURAL CHEMICAL ANALYSIS
YEASTS
550200* - Biochemistry
550400 - Genetics