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Title: Effects of alcohol on pulsatile luteinizing hormone (LH) and follicle stimulating hormone (FSH) secretion in the adult male rat

Abstract

To determine possible hypothalamic actions of alcohol on hormone secretion, the effects of acute intragastric alcohol on plasma LH and FSH pulsations were studied. One jugular and one intragastric cannula were surgically implanted into adult male Sprague Dawley rats. Eight days later, rats were bilaterally castrated at 1400 h and infused intragastrically with either saline or 3 g/kg ethanol between 0700 h 0800 h the next days. Blood samples (300 microliters) were collected every 5 min for 3 h (starting at 0800 h), centrifuged and the plasma was frozen for LH and FSH radioimmunoassay. The blood cells were resuspended in saline and returned to the animal immediately following the next sample collection. While the mean plasma LH or FSH concentration did not vary significantly between the alcohol-treated and saline-treated rats, the mean LH (but not FSH) pulse frequency was lower in ethanol-treated rats (3.3 {plus minus} 0.25 pulses/3 h) than saline-treated controls (7.2 {plus minus} 0.3 pulses/3 h). In addition, mean area under the OH pulses were significantly greater in ethanol-treated than saline controls. These data suggest that: (1) ethanol acts to reduce the frequency of LHRH release for the hypothalamus and increase the area under each LH pulse; andmore » (2) LH and FSH secretion are differentially regulated.« less

Authors:
; ;  [1]
  1. Univ. of Arkansas for Medical Sciences, Little Rock (USA)
Publication Date:
OSTI Identifier:
6831796
Resource Type:
Journal Article
Journal Name:
FASEB Journal (Federation of American Societies for Experimental Biology); (USA)
Additional Journal Information:
Journal Volume: 3:3; Journal ID: ISSN 0892-6638
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; ETHANOL; TOXICITY; FSH; BIOCHEMICAL REACTION KINETICS; LH; RADIOIMMUNOASSAY; RATS; ALCOHOLS; ANIMALS; BIOASSAY; DIAGNOSTIC TECHNIQUES; GONADOTROPINS; HORMONES; HYDROXY COMPOUNDS; IMMUNOASSAY; IMMUNOLOGY; ISOTOPE APPLICATIONS; KINETICS; MAMMALS; ORGANIC COMPOUNDS; PEPTIDE HORMONES; PITUITARY HORMONES; RADIOASSAY; RADIOIMMUNODETECTION; RADIOIMMUNOLOGY; REACTION KINETICS; RODENTS; TRACER TECHNIQUES; VERTEBRATES; 560300* - Chemicals Metabolism & Toxicology; 550201 - Biochemistry- Tracer Techniques

Citation Formats

Badger, T M, Abdallah, M M, and Hayden, J B. Effects of alcohol on pulsatile luteinizing hormone (LH) and follicle stimulating hormone (FSH) secretion in the adult male rat. United States: N. p., 1989. Web.
Badger, T M, Abdallah, M M, & Hayden, J B. Effects of alcohol on pulsatile luteinizing hormone (LH) and follicle stimulating hormone (FSH) secretion in the adult male rat. United States.
Badger, T M, Abdallah, M M, and Hayden, J B. 1989. "Effects of alcohol on pulsatile luteinizing hormone (LH) and follicle stimulating hormone (FSH) secretion in the adult male rat". United States.
@article{osti_6831796,
title = {Effects of alcohol on pulsatile luteinizing hormone (LH) and follicle stimulating hormone (FSH) secretion in the adult male rat},
author = {Badger, T M and Abdallah, M M and Hayden, J B},
abstractNote = {To determine possible hypothalamic actions of alcohol on hormone secretion, the effects of acute intragastric alcohol on plasma LH and FSH pulsations were studied. One jugular and one intragastric cannula were surgically implanted into adult male Sprague Dawley rats. Eight days later, rats were bilaterally castrated at 1400 h and infused intragastrically with either saline or 3 g/kg ethanol between 0700 h 0800 h the next days. Blood samples (300 microliters) were collected every 5 min for 3 h (starting at 0800 h), centrifuged and the plasma was frozen for LH and FSH radioimmunoassay. The blood cells were resuspended in saline and returned to the animal immediately following the next sample collection. While the mean plasma LH or FSH concentration did not vary significantly between the alcohol-treated and saline-treated rats, the mean LH (but not FSH) pulse frequency was lower in ethanol-treated rats (3.3 {plus minus} 0.25 pulses/3 h) than saline-treated controls (7.2 {plus minus} 0.3 pulses/3 h). In addition, mean area under the OH pulses were significantly greater in ethanol-treated than saline controls. These data suggest that: (1) ethanol acts to reduce the frequency of LHRH release for the hypothalamus and increase the area under each LH pulse; and (2) LH and FSH secretion are differentially regulated.},
doi = {},
url = {https://www.osti.gov/biblio/6831796}, journal = {FASEB Journal (Federation of American Societies for Experimental Biology); (USA)},
issn = {0892-6638},
number = ,
volume = 3:3,
place = {United States},
year = {Thu Feb 09 00:00:00 EST 1989},
month = {Thu Feb 09 00:00:00 EST 1989}
}