Partial separation of platelet and placental adenosine receptors from adenosine A2-like binding protein

Journal Article · Sun Apr 01 00:00:00 EST 1990 · Molecular Pharmacology; (USA)

OSTI ID:6828305

Zolnierowicz, S; Work, C; Hutchison, K; Fox, I H ^[1]

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1. University Hospital, Ann Arbor, MI (USA)

The ubiquitous adenosine A2-like binding protein obscures the binding properties of adenosine receptors assayed with 5'-N-({sup 3}H)ethylcarboxamidoadenosine (({sup 3}H)NECA). To solve this problem, we developed a rapid and simple method to separate adenosine receptors from the adenosine A2-like binding protein. Human platelet and placental membranes were solubilized with 1% 3-((3-cholamidopropyl)dimethylammonio)-1-propanesulfonate. The soluble platelet extract was precipitated with polyethylene glycol and the fraction enriched in adenosine receptors was isolated from the precipitate by differential centrifugation. The adenosine A2-like binding protein was removed from the soluble placental extract with hydroxylapatite and adenosine receptors were precipitated with polyethylene glycol. The specificity of the ({sup 3}H)NECA binding is typical of an adenosine A2 receptor for platelets and an adenosine A1 receptor for placenta. This method leads to enrichment of adenosine A2 receptors for platelets and adenosine A1 receptors for placenta. This provides a useful preparation technique for pharmacologic studies of adenosine receptors.

• OSTI ID: 6828305
• Journal Information: Molecular Pharmacology; (USA), Vol. 37:4; ISSN 0026-895X
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ADENOSINE
RECEPTORS
FRACTIONATION
BIOCHEMICAL REACTION KINETICS
BLOOD PLATELETS
CELL MEMBRANES
MAN
PLACENTA
RADIOASSAY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ULTRACENTRIFUGATION
VASODILATORS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARDIOVASCULAR AGENTS
CELL CONSTITUENTS
CENTRIFUGATION
DRUGS
FETAL MEMBRANES
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MEMBRANES
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
REACTION KINETICS
RIBOSIDES
SEPARATION PROCESSES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques