High speed flow cytometric detection of rare glycophorin A mutations in human blood cells
- Lawrence Livermore National Lab., CA (United States)
The glycophorin A (GPA) assay utilizes immunofluorescent labeling and flow cytometry to measure the frequency of peripheral erythrocytes with mutant phenotypes, presumably due to mutations in erythroid precursor cells. Analysis of 5 [times] 10[sup 6] cells/assay is used to enumerate variant erythrocytes that occur at a frequency of 3-10 [times] 10[sup [minus]6] in unexposed donors. Extension of this assay to human reticulocytes requires detection of variants that occur at frequencies as low as 3 [times] 10[sup [minus]8]. The authors have used high speed data acquisition and cell classification electronics to perform 3-color analysis at rates up to 20,000 cells/s. High speed analysis of up to 10[sup 8] cells/assay has been used to enumerate GPA-variant reticulocytes in normal donors.
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 6827969
- Report Number(s):
- CONF-9303114-; CODEN: CYTODQ
- Journal Information:
- Cytometry (Baltimore); (United States), Vol. 6; Conference: 16. congress of the International Society for Analytical Cytology, Colorado Springs, CO (United States), 21-26 Mar 1993; ISSN 0196-4763
- Country of Publication:
- United States
- Language:
- English
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