Human papilloma virus DNAs immortalize normal human mammary epithelial cells and reduce their growth factor requirements

Journal Article · Mon Jan 01 00:00:00 EST 1990 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.87.1.463· OSTI ID:6827801

Band, V; Zajchowski, D; Kulesa, V; Sager, R ^[1]

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1. Dana-Farber Cancer Institute, Boston, MA (USA)

Human papilloma virus (HPV) types 16 and 18 are most commonly associated with cervical carcinoma in patients and induce immortalization of human keratinocytes in culture. HPV has not been associated with breast cancer. This report describes the immortalization of normal human mammary epithelial cells (76N) by plasmid pHPV18 or pHPV16, each containing the linearized viral genome. Transfectants were grown continuously for more than 60 passages, whereas 76N cells senesce after 18-20 passages. The transfectants also differ from 76N cells in cloning in a completely defined medium called D2 and growing a minimally supplemented defined medium (D3) containing epidermal growth factor. All transfectant tested contain integrated HPV DNA, express HPV RNA, and produce HPV E7 protein. HPV transfectants do not form tumors in a nude mouse assay. It is concluded that products of the HPV genome induce immortalization of human breast epithelial cells and reduce their growth factor requirements. This result raises the possibility that HPV might be involved in breast cancer. Furthermore, other tissue-specific primary epithelial cells that are presently difficult to grown and investigate may also be immortalized by HPV.

• OSTI ID: 6827801
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 87:1; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
EPITHELIUM
ONCOGENIC TRANSFORMATIONS
ONCOGENIC VIRUSES
DNA HYBRIDIZATION
ACTIN
CARCINOGENESIS
CARCINOMAS
DNA
GROWTH
MAMMARY GLANDS
MESSENGER-RNA
METHIONINE
MORPHOLOGY
PATIENTS
PHOSPHORUS 32
PLASMIDS
SULFUR 35
AMINO ACIDS
ANIMAL TISSUES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL TRANSFORMATIONS
DAYS LIVING RADIOISOTOPES
DISEASES
DRUGS
EVEN-ODD NUCLEI
GLANDS
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MICROORGANISMS
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PARASITES
PATHOGENESIS
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RNA
SULFUR ISOTOPES
TISSUES
VIRUSES
550401* - Genetics- Tracer Techniques