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Title: Mapping of a human A2a adenosine receptor (ADORA2) to chromosome 22

Abstract

Using a probe derived from the recently cloned rat A2a receptor A2aR, a panel containing multiple human-rodent somatic cell hybrids was probed by Southern hybridization. A faint but specific signal was seen in the chromosome 22-only line. A human chromosome 22 cosmid library (LL22NC02, Lawrence Livermore Library) was then screened by Southern hybridization to the same probe; two cosmid clones (54C4 and 30D1) were isolated. Several restriction digests of both cosmids were rehybridized with the rat A2aR probe, and a 700-bp PstI fragment that specifically hybridized to the probe was subsequently cloned into plasmid Bluescript II. This subclone, designated pHUAD2, was then hybridized to a panel of 60 human-rodent somatic cell hybrid lines digested with SacI to address the possibility of related genes or pseudogenes located on other chromosomes. Two human bands of approximately 3 and 4 kb were seen in a total of four hybrid lines; two of these lines contain only chromosome 22. Although a previous report had suggested that the human A2a receptor resided on human chromosome 11 with a related sequence on human chromosome 11 with a related sequence on human chromosome 10, hybrids containing human chromosomes 10 and 11 did not hybridize to the probe.

Authors:
; ; ; ;  [1]
  1. Massachusetts General Hospital, Charlestown, MA (United States)
Publication Date:
OSTI Identifier:
6822593
Resource Type:
Journal Article
Journal Name:
Genomics; (United States)
Additional Journal Information:
Journal Volume: 20:2; Journal ID: ISSN 0888-7543
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; HUMAN CHROMOSOME 22; GENETIC MAPPING; RECEPTORS; GENES; DNA HYBRIDIZATION; DNA-CLONING; CHROMOSOMES; CLONING; HUMAN CHROMOSOMES; HYBRIDIZATION; MAPPING; MEMBRANE PROTEINS; ORGANIC COMPOUNDS; PROTEINS; 550400* - Genetics

Citation Formats

MacCollin, M, MacDonald, M, Gusella, J, Peterfreund, R, and Fink, J S. Mapping of a human A2a adenosine receptor (ADORA2) to chromosome 22. United States: N. p., 1994. Web. doi:10.1006/geno.1994.1181.
MacCollin, M, MacDonald, M, Gusella, J, Peterfreund, R, & Fink, J S. Mapping of a human A2a adenosine receptor (ADORA2) to chromosome 22. United States. https://doi.org/10.1006/geno.1994.1181
MacCollin, M, MacDonald, M, Gusella, J, Peterfreund, R, and Fink, J S. Tue . "Mapping of a human A2a adenosine receptor (ADORA2) to chromosome 22". United States. https://doi.org/10.1006/geno.1994.1181.
@article{osti_6822593,
title = {Mapping of a human A2a adenosine receptor (ADORA2) to chromosome 22},
author = {MacCollin, M and MacDonald, M and Gusella, J and Peterfreund, R and Fink, J S},
abstractNote = {Using a probe derived from the recently cloned rat A2a receptor A2aR, a panel containing multiple human-rodent somatic cell hybrids was probed by Southern hybridization. A faint but specific signal was seen in the chromosome 22-only line. A human chromosome 22 cosmid library (LL22NC02, Lawrence Livermore Library) was then screened by Southern hybridization to the same probe; two cosmid clones (54C4 and 30D1) were isolated. Several restriction digests of both cosmids were rehybridized with the rat A2aR probe, and a 700-bp PstI fragment that specifically hybridized to the probe was subsequently cloned into plasmid Bluescript II. This subclone, designated pHUAD2, was then hybridized to a panel of 60 human-rodent somatic cell hybrid lines digested with SacI to address the possibility of related genes or pseudogenes located on other chromosomes. Two human bands of approximately 3 and 4 kb were seen in a total of four hybrid lines; two of these lines contain only chromosome 22. Although a previous report had suggested that the human A2a receptor resided on human chromosome 11 with a related sequence on human chromosome 11 with a related sequence on human chromosome 10, hybrids containing human chromosomes 10 and 11 did not hybridize to the probe.},
doi = {10.1006/geno.1994.1181},
url = {https://www.osti.gov/biblio/6822593}, journal = {Genomics; (United States)},
issn = {0888-7543},
number = ,
volume = 20:2,
place = {United States},
year = {1994},
month = {3}
}