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Title: Physical mapping of chromosome 12q breakpoints in lipoma, pleomorphic salivary gland adenoma, uterine leiomyoma, and myxoid liposarcoma

Abstract

The authors report here the physical mapping of recurrent chromosome 12q13-q15 breakpoints in cell lines derived from primary myxoid liposarcoma, lipoma, uterine leiomyoma, and pleomorphic adenoma of the salivary glands. In fluorescence in situ hybridization (FISH) experiments, they first mapped the position of the chromosome 12 translocation breakpoint in uterine leiomyoma cell line LM-30.1/SV40 relative to loci COL2A1, D12S4, D12S17, D12S6, D12S19, D12S8, and D12S7. It mapped between linkage probes CRI-C86 (D12S19) and p7G11 (D12S8). They then isolated YAC clones using CRI-C86- and p7G11-derived sequence-tagged sites, constructed corresponding YAC contigs of 310 and 800 kb, respectively, and a mixture of them was used to routinely study the various tumor cell lines by FISH analysis. The chromosome 12 breakpoints of all tumor cell lines tested mapped between cosmids LLNL12NCO1-98C10 and LLNL12NCO1-113D12. None of the breakpoints appeared to map within any of the isolated YAC clones. Furthermore, FISH analysis using cosmid LLNL12-NCO1-144G3, which maps at the CHOP locus, revealed that the chromosome 12 breakpoints in all cell lines of the three benign solid tumors that were tested were located distal to the chromosome 12 translocation breakpoint with the CHOP gene in myxoid liposarcoma cells with t(12;16). In conclusion, the studies seem tomore » indicate that the chromosome 12 breakpoints of myxoid liposarcoma, lipoma, uterine leiomyoma, and pleomorphic adenoma of the salivary glands are all clustered within the 7-cM interval between D12S19 and D12S8, with those of the benign solid tumors distal to CHOP. Finally, the MYF5 gene mapped telomeric to LLNL12NCO1-113D12, and the MIP gene mapped centromeric to the chromosome 12 translocation breakpoint in myxoid liposarcoma cells. 56 refs., 5 figs., 3 tabs.« less

Authors:
; ; ; ; ;  [1]; ; ;  [2];  [3]
  1. (Univ. of Leuven (Belgium))
  2. (Univ. of Bremen (Germany))
  3. (Roswell Memorial Institute, Buffalo, NY (United States))
Publication Date:
OSTI Identifier:
6821601
Alternate Identifier(s):
OSTI ID: 6821601
Resource Type:
Journal Article
Journal Name:
Genomics; (United States)
Additional Journal Information:
Journal Volume: 20:2; Journal ID: ISSN 0888-7543
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; HUMAN CHROMOSOME 12; GENETIC MAPPING; NEOPLASMS; GENES; CHROMOSOMAL ABERRATIONS; DNA HYBRIDIZATION; MUSCLES; SARCOMAS; UTERUS; BODY; CHROMOSOMES; DISEASES; FEMALE GENITALS; HUMAN CHROMOSOMES; HYBRIDIZATION; MAPPING; MUTATIONS; ORGANS 550400* -- Genetics

Citation Formats

Schoenmakers, E.F.P.M., Kools, P.F.J., Mols, R., Cin, P.D., Van Den Berghe, H., Van De Ven, W.J.M., Kazmierczak, B., Bartnitzke, S., Bullerdiek, J., and De Jong, P.J. Physical mapping of chromosome 12q breakpoints in lipoma, pleomorphic salivary gland adenoma, uterine leiomyoma, and myxoid liposarcoma. United States: N. p., 1994. Web. doi:10.1006/geno.1994.1155.
Schoenmakers, E.F.P.M., Kools, P.F.J., Mols, R., Cin, P.D., Van Den Berghe, H., Van De Ven, W.J.M., Kazmierczak, B., Bartnitzke, S., Bullerdiek, J., & De Jong, P.J. Physical mapping of chromosome 12q breakpoints in lipoma, pleomorphic salivary gland adenoma, uterine leiomyoma, and myxoid liposarcoma. United States. doi:10.1006/geno.1994.1155.
Schoenmakers, E.F.P.M., Kools, P.F.J., Mols, R., Cin, P.D., Van Den Berghe, H., Van De Ven, W.J.M., Kazmierczak, B., Bartnitzke, S., Bullerdiek, J., and De Jong, P.J. Tue . "Physical mapping of chromosome 12q breakpoints in lipoma, pleomorphic salivary gland adenoma, uterine leiomyoma, and myxoid liposarcoma". United States. doi:10.1006/geno.1994.1155.
@article{osti_6821601,
title = {Physical mapping of chromosome 12q breakpoints in lipoma, pleomorphic salivary gland adenoma, uterine leiomyoma, and myxoid liposarcoma},
author = {Schoenmakers, E.F.P.M. and Kools, P.F.J. and Mols, R. and Cin, P.D. and Van Den Berghe, H. and Van De Ven, W.J.M. and Kazmierczak, B. and Bartnitzke, S. and Bullerdiek, J. and De Jong, P.J.},
abstractNote = {The authors report here the physical mapping of recurrent chromosome 12q13-q15 breakpoints in cell lines derived from primary myxoid liposarcoma, lipoma, uterine leiomyoma, and pleomorphic adenoma of the salivary glands. In fluorescence in situ hybridization (FISH) experiments, they first mapped the position of the chromosome 12 translocation breakpoint in uterine leiomyoma cell line LM-30.1/SV40 relative to loci COL2A1, D12S4, D12S17, D12S6, D12S19, D12S8, and D12S7. It mapped between linkage probes CRI-C86 (D12S19) and p7G11 (D12S8). They then isolated YAC clones using CRI-C86- and p7G11-derived sequence-tagged sites, constructed corresponding YAC contigs of 310 and 800 kb, respectively, and a mixture of them was used to routinely study the various tumor cell lines by FISH analysis. The chromosome 12 breakpoints of all tumor cell lines tested mapped between cosmids LLNL12NCO1-98C10 and LLNL12NCO1-113D12. None of the breakpoints appeared to map within any of the isolated YAC clones. Furthermore, FISH analysis using cosmid LLNL12-NCO1-144G3, which maps at the CHOP locus, revealed that the chromosome 12 breakpoints in all cell lines of the three benign solid tumors that were tested were located distal to the chromosome 12 translocation breakpoint with the CHOP gene in myxoid liposarcoma cells with t(12;16). In conclusion, the studies seem to indicate that the chromosome 12 breakpoints of myxoid liposarcoma, lipoma, uterine leiomyoma, and pleomorphic adenoma of the salivary glands are all clustered within the 7-cM interval between D12S19 and D12S8, with those of the benign solid tumors distal to CHOP. Finally, the MYF5 gene mapped telomeric to LLNL12NCO1-113D12, and the MIP gene mapped centromeric to the chromosome 12 translocation breakpoint in myxoid liposarcoma cells. 56 refs., 5 figs., 3 tabs.},
doi = {10.1006/geno.1994.1155},
journal = {Genomics; (United States)},
issn = {0888-7543},
number = ,
volume = 20:2,
place = {United States},
year = {1994},
month = {3}
}