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Title: Functional assessment of hepatocytes after transplantation into rat spleen

Abstract

The retention of structural integrity and metabolic function by isolated hepatocytes after ectopic transplantation has been investigated in autografted rats. Rats were partially hepatectomized and isolated hepatocytes prepared from the excised liver lobes were implanted into their spleens. Histological examination of the spleens 7 or more weeks after implantation revealed aggregates of hepatocytes in the red pulp. Two tests of biochemical function were applied to the hepatocytes after tranplantation. In the first the hepatobiliary imaging agent technetium-99m N-(N'-(2,6-dimethylphenyl)carbamoylmethyl)iminodiacetic acid (/sup 99//sup m/Tc HIDA), which was shown to be avidly taken up by isolated hepatocytes in vitro, was infused into the tail veins of autograft and control rats. Radioactivity accumulating in the spleens of autografted rats was markedly greater than that in controls implanted with lethally damaged cells or in nontransplanted rats. In the second the presence of bilirubin metabolites was sought in autograft spleens after intravenous infusion of bilirubin. Both mono- and diglucuronides of bilirubin were recovered from the spleens of autograft rats but no conjugates were recovered from the spleens of unoperated controls. We conclude that after autotransplantation isolated hepatocytes retain their morphology and at least some of their functional activities.

Authors:
; ; ; ;
Publication Date:
Research Org.:
Royal Free Hospital School of Medicine, London, England
OSTI Identifier:
6816634
Alternate Identifier(s):
OSTI ID: 6816634
Resource Type:
Journal Article
Journal Name:
Transplantation; (United States)
Additional Journal Information:
Journal Volume: 33:2
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; 59 BASIC BIOLOGICAL SCIENCES; BILIRUBIN; METABOLISM; LIVER CELLS; TRANSPLANTS; SPLEEN; BIOLOGICAL FUNCTIONS; TECHNETIUM 99; BIOLOGICAL ACCUMULATION; HEPATECTOMY; HISTOLOGY; IMINES; ISOMERIC NUCLEI; PERTECHNETATES; RATS; ANIMAL CELLS; ANIMALS; AZOLES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; CARBOXYLIC ACIDS; FUNCTIONS; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; HOURS LIVING RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; MAMMALS; MEDICINE; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; OXYGEN COMPOUNDS; PIGMENTS; PYRROLES; RADIOISOTOPES; RODENTS; SOMATIC CELLS; SURGERY; TECHNETIUM COMPOUNDS; TECHNETIUM ISOTOPES; TRANSITION ELEMENT COMPOUNDS; VERTEBRATES; YEARS LIVING RADIOISOTOPES 550601* -- Medicine-- Unsealed Radionuclides in Diagnostics; 551001 -- Physiological Systems-- Tracer Techniques

Citation Formats

Woods, R.J., Fuller, B.J., Attenburrow, V.D., Nutt, L.H., and Hobbs, K.E.F. Functional assessment of hepatocytes after transplantation into rat spleen. United States: N. p., 1982. Web. doi:10.1097/00007890-198202000-00004.
Woods, R.J., Fuller, B.J., Attenburrow, V.D., Nutt, L.H., & Hobbs, K.E.F. Functional assessment of hepatocytes after transplantation into rat spleen. United States. doi:10.1097/00007890-198202000-00004.
Woods, R.J., Fuller, B.J., Attenburrow, V.D., Nutt, L.H., and Hobbs, K.E.F. Mon . "Functional assessment of hepatocytes after transplantation into rat spleen". United States. doi:10.1097/00007890-198202000-00004.
@article{osti_6816634,
title = {Functional assessment of hepatocytes after transplantation into rat spleen},
author = {Woods, R.J. and Fuller, B.J. and Attenburrow, V.D. and Nutt, L.H. and Hobbs, K.E.F.},
abstractNote = {The retention of structural integrity and metabolic function by isolated hepatocytes after ectopic transplantation has been investigated in autografted rats. Rats were partially hepatectomized and isolated hepatocytes prepared from the excised liver lobes were implanted into their spleens. Histological examination of the spleens 7 or more weeks after implantation revealed aggregates of hepatocytes in the red pulp. Two tests of biochemical function were applied to the hepatocytes after tranplantation. In the first the hepatobiliary imaging agent technetium-99m N-(N'-(2,6-dimethylphenyl)carbamoylmethyl)iminodiacetic acid (/sup 99//sup m/Tc HIDA), which was shown to be avidly taken up by isolated hepatocytes in vitro, was infused into the tail veins of autograft and control rats. Radioactivity accumulating in the spleens of autografted rats was markedly greater than that in controls implanted with lethally damaged cells or in nontransplanted rats. In the second the presence of bilirubin metabolites was sought in autograft spleens after intravenous infusion of bilirubin. Both mono- and diglucuronides of bilirubin were recovered from the spleens of autograft rats but no conjugates were recovered from the spleens of unoperated controls. We conclude that after autotransplantation isolated hepatocytes retain their morphology and at least some of their functional activities.},
doi = {10.1097/00007890-198202000-00004},
journal = {Transplantation; (United States)},
number = ,
volume = 33:2,
place = {United States},
year = {1982},
month = {2}
}