In vitro effects of OK-432 on irradiated mouse bone marrow cells

Journal Article · Wed Jun 15 00:00:00 EDT 1994 · International Journal of Radiation Oncology, Biology and Physics; (United States)

DOI:https://doi.org/10.1016/0360-3016(94)90471-5· OSTI ID:6809632

Nose, Masako; Kawase, Yoshiko; Suzuki, Gen; Akashi, Makoto; Akanuma, Atsuo ^[1]; Aoki, Yoshiro ^[2]

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1. National Institute of Radiological Sciences, Chiba (Japan)
2. Univ. of Tokyo (Japan)

In vitro effects of OK-432 (polysaccharide extract of Streptococcus haemolyticus) on irradiated mouse bone marrow cells are examined. Bone marrow cells of BDF1 mouse (1 [times] 10[sup 6] cells/ml) were incubated with alpha medium, 2% fetal calf serum and OK-432 in a CO[sub 2] incubator at 37[degrees]C for 24, 48 and 72 h, respectively. After centrifugation, each supernatant was collected and used for conditioned medium in a CFU-GM assay: Changes in CFU-GM as a function of incubation time and OK-432 dose were examined; changes of CFU-GM according to various doses of OK-432 were examined in two mouse strains, BDF[sub 1] and BALB/c mouse; changes in the protective effect of OK-432 in terms of CFU-GM as a function of administration timing of OK-432 in relation to irradiation. As a radiation source, [sup 137]Cs at a dose rate of 500 cGy/min was used. The CFU-GM decreased with the incubation time when OK-432 was not administered, while it significantly increased with incubation time when OK-432 was added at 0.5 and 1.0 KE/ml at 48-72 h of incubation. The former showed marked increase at 48-72 h of incubation. CFU-GM of BDF[sub 1] mouse was always higher than that of BALB/c mouse for any dose of OK-432. CFU-GM per femur according to the timing of administration of OK-432 from 24 h before to 24 h after irradiation showed 10299 [+-] 2300 (24 h before), 10783 [+-] 2463 (3 h before), 10045 [+-] 1501 (immediately after), 8504 [+-] 1188 (3 h after), 4898 [+-] 1212 (6 h after), 1214 [+-] 736 (12 h after) and 181 [+-] 113 (24 h after irradiation), respectively. OK-432 stimulates cultures mouse bone marrow cells to produce GM-CSF in vitro by direct contact action. This direct stimulating action of OK-432 on GM-CSF production of bone marrow cells can be kept from 24 h before to at least 3 h after irradiation. 6 refs., 4 figs.

• OSTI ID: 6809632
• Journal Information: International Journal of Radiation Oncology, Biology and Physics; (United States), Vol. 29:3; ISSN 0360-3016
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BONE MARROW CELLS
RADIOSENSITIVITY
POLYSACCHARIDES
RADIOPROTECTIVE SUBSTANCES
ANIMAL CELLS
CARBOHYDRATES
CONNECTIVE TISSUE CELLS
DRUGS
ORGANIC COMPOUNDS
SACCHARIDES
SOMATIC CELLS
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture