Quantitation of cell concentration using the flow cytometer

Conference · Fri Jan 01 00:00:00 EST 1982 · Cytometry (Baltimore); (United States)

OSTI ID:6808048

Stewart, C C; Steinkamp, J A

A simple procedure has been developed to simultaneously measure cell concentration and analyze marker positive cell populations using the flow cytometer. Fluorescent latex particles of known concentration are added to the sample to be analyzed. Marker positive cells and fluorescent particles are then analyzed using flow cytometric methods. Since the particle concentration is known, the number of particles that have accumulated gives the exact volume of the sample analyzed. The concentration of total and marker positive cells that were analyzed then can be calculated. The method will be illustrated using single, dual, and triple laser excitation of cells stained for DNA content in combination with green or red fluorescent spheres. The usefulness of the procedure is demonstrated by counting cells from highly necrotic tumors where large amounts of debris are present.

• Research Organization: Los Alamos National Lab., NM
• OSTI ID: 6808048
• Journal Information: Cytometry (Baltimore); (United States), Vol. 2:4; Conference: Analytical cytology conference VIII, Wentworth-by-the-Sea, NH, USA, 19 May 1981
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
DNA
QUANTITATIVE CHEMICAL ANALYSIS
TUMOR CELLS
MEASURING METHODS
CALCULATION METHODS
CELL FLOW SYSTEMS
COMPARATIVE EVALUATIONS
CYTOLOGY
FLUORESCENCE
NECROSIS
PARTICLES
PATHOLOGY
QUANTITY RATIO
RADIOINDUCTION
ANIMAL CELLS
BIOLOGY
CHEMICAL ANALYSIS
LUMINESCENCE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
550300* - Cytology
400102 - Chemical & Spectral Procedures