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Title: Submicrogram determination of lanthanides through quenching of calcein blue fluorescence

Abstract

It has been found that trace levels of lanthanide ions efficiently quench the fluorescence of calcein blue and that an analytical method based on this quenching is far more sensitive (0.01-0.02 ..mu..g/mL depending on the identity of the lanthanide ion) than existing methods based on absorption spectrophotometry. The sensitivity levels are comparable to those noted for fluorescence observation of the few lanthanides which luminesce directly, but the calcein blue method may be applied equally well to any lanthanide ion. Interference by divalent transition-metal ions has been noted, but the lower degrees of quenching efficiency by these ions ensure that significant interference will exist only at relatively high metal levels.

Authors:
Publication Date:
Research Org.:
Squibb Institute for Medical Research, New Brunswick, NJ
OSTI Identifier:
6804048
Resource Type:
Journal Article
Journal Name:
Anal. Chem.; (United States)
Additional Journal Information:
Journal Volume: 59:8
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; CERIUM; FLUORESCENCE SPECTROSCOPY; COBALT; COPPER; DYSPROSIUM; ERBIUM; EUROPIUM; GADOLINIUM; HOLMIUM; IRON; LANTHANUM; LUTETIUM; MANGANESE; NEODYMIUM; NICKEL; PRASEODYMIUM; SAMARIUM; TERBIUM; THULIUM; YTTERBIUM; ZINC; EXPERIMENTAL DATA; INTERFERING ELEMENTS; QUANTITATIVE CHEMICAL ANALYSIS; RADIATIONLESS DECAY; TRACE AMOUNTS; CHEMICAL ANALYSIS; DATA; DE-EXCITATION; ELEMENTS; EMISSION SPECTROSCOPY; ENERGY TRANSFER; ENERGY-LEVEL TRANSITIONS; INFORMATION; METALS; NUMERICAL DATA; RARE EARTHS; SPECTROSCOPY; TRANSITION ELEMENTS; 400102* - Chemical & Spectral Procedures

Citation Formats

Brittain, H G. Submicrogram determination of lanthanides through quenching of calcein blue fluorescence. United States: N. p., 1987. Web. doi:10.1021/ac00135a012.
Brittain, H G. Submicrogram determination of lanthanides through quenching of calcein blue fluorescence. United States. https://doi.org/10.1021/ac00135a012
Brittain, H G. 1987. "Submicrogram determination of lanthanides through quenching of calcein blue fluorescence". United States. https://doi.org/10.1021/ac00135a012.
@article{osti_6804048,
title = {Submicrogram determination of lanthanides through quenching of calcein blue fluorescence},
author = {Brittain, H G},
abstractNote = {It has been found that trace levels of lanthanide ions efficiently quench the fluorescence of calcein blue and that an analytical method based on this quenching is far more sensitive (0.01-0.02 ..mu..g/mL depending on the identity of the lanthanide ion) than existing methods based on absorption spectrophotometry. The sensitivity levels are comparable to those noted for fluorescence observation of the few lanthanides which luminesce directly, but the calcein blue method may be applied equally well to any lanthanide ion. Interference by divalent transition-metal ions has been noted, but the lower degrees of quenching efficiency by these ions ensure that significant interference will exist only at relatively high metal levels.},
doi = {10.1021/ac00135a012},
url = {https://www.osti.gov/biblio/6804048}, journal = {Anal. Chem.; (United States)},
number = ,
volume = 59:8,
place = {United States},
year = {Wed Apr 15 00:00:00 EDT 1987},
month = {Wed Apr 15 00:00:00 EDT 1987}
}