The human gene for xeroderma pigmentosum complementation group G (XPG) maps to 13q33 by fluorescence in situ hybridization

Journal Article · Sun May 01 00:00:00 EDT 1994 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1994.1261· OSTI ID:6792792

Samec, S; Corlet, J; Scherly, D; Clarkson, S G ^[1]; Jones, T A; Sheer, D ^[2]; Wood, R D ^[3]

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1. Centre Medical Universitaire, Geneva (Switzerland)
2. Imperial Cancer Research Fund, London (United Kingdom)
3. Clare Hall Labs., Hertfordshire (United Kingdom)

Recently, a human cDNA was isolated that restores normal levels of UV resistance and DNA repair synthesis when expressed in vivo in a lymphoblastoid cell line representing XP group G. The XP-G complementing gene (XPG) generates an mRNA of [approximately]4 kb and encodes a protein (XPGC) with homology to the RAD2 DNA repair protein of Saccharomyces cerevisiae. One hundred twenty nanograms of labeled probe was mixed with 2 [mu]g of human C[sub 0]t-1 DNA as a competitor for repetitive elements. Denaturation, dehydration, hybridization, and washing were performed as described. Probe detection was achieved by incubating metaphase spreads sequentially with 2 [mu]g/ml avidin-Texas Red, 5 [mu]g/ml biotinylated goat anti-avidin antibody, and 2 [mu]g/ml avidin-Texas Red. R-banding was revealed after incubation with fluorescein-labeled anti-BrdU mouse monoclonal antibody. Chromosomes were counter-stained with 0.06 [mu]g/ml DAPI in Citifluor. Analysis of 40 metaphase spreads showed paired signals on both copies of chromosome 13 at band 13q33. No other paired signals were seen consistently on any other chromosome. 9 refs., 1 fig.

• OSTI ID: 6792792
• Journal Information: Genomics; (United States), Vol. 21:1; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HUMAN CHROMOSOME 13
GENETIC MAPPING
XERODERMA PIGMENTOSUM
ETIOLOGY
DNA HYBRIDIZATION
DNA-CLONING
BIOTECHNOLOGY
CHROMOSOMES
CLONING
CONGENITAL DISEASES
DISEASES
GENETIC ENGINEERING
HEREDITARY DISEASES
HUMAN CHROMOSOMES
HYBRIDIZATION
MAPPING
NUCLEIC ACID HYBRIDIZATION
SKIN DISEASES
550400* - Genetics