Assignment of the gene for the human proliferating cell nucleolar protein P120 (NOL1) to chromosome 12p13 by fluorescence in situ hybridization and polymerase chain reaction with somatic cell hybrids
- Center for Human Genetics, Leuven (Belgium)
- Univ. of Leuven (Belgium)
The human NOL1 protein is a proliferation associated antigen that is temporally regulated during the cell cycle with a dramatic increase in protein expression at the G1-S boundary, suggesting that NOL1 may play a role in the regulation of the cell cycle and the increased nucleolar activity that is associated with the cell proliferation. The authors have isolated a cosmid (LL12NCO1N147G1) for NOL1 from the chromosome 12-specific cosmid library LL12NCO1, constructed at the Biomedical Sciences Division, Lawrence Livermore Laboratory (Livermore, CA) and obtained from P. de Jong. The identity of the cosmid was confirmed by restriction mapping and PCR. Fluorescence in situ hybridization (FISH) was performed on metaphase chromosomes of normal lymphocytes with the cosmid 147G1 and a probe for the alphoid centromeric region of chromosome 12, developed by U. Weier. Probe labeling and in situ hybridization were performed as described. The FISH results showed that NOL1 maps to the distal part of chromosome 12p. 6 refs., 1 fig.
- OSTI ID:
- 6792645
- Journal Information:
- Genomics; (United States), Vol. 21:1; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
Similar Records
A fluorescence in situ hybridization map of human chromosome 21 consisting of 30 genetic and physical markers on the chromosome: Localization of 137 additional YAC and cosmid clones with respect to this map
Localization of the gene for DNA polymerase [epsilon] (POLE) to human chromosome 12q24. 3 and rat chromosome 12 by somatic cell hybrid panels and fluorescence in situ hybridization