Chromosome number9 specific repetitive DNA sequence

Conference · Thu May 01 00:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6791517

Joste, N E; Cram, L S; Hildebrand, C E; Jones, M; Longmire, J; Robinson, T; Moyzis, R K

Human repetitive DNA libraries have been constructed and various recombinant DNA clones isolated that are likely candidates for chromosome specific sequences. The first clone tested (pHuR 98; plasmid human repeat 98) was biotinylated and hybridized to human chromosomes in situ. The hybridized recombinant probe was detected with fluoresceinated avidin, and chromosomes were counter-stained with either propidium iodide or distamycin-DAPI. Specific hybridization to chromosome band 9q1 was obtained. The localization was confirmed by hybridizing radiolabeled pHuR 98 DNA to human chromosomes sorted by flow cytometry. Various methods, including orthogonal field pulsed gel electrophoresis analysis indicate that 75 kilobase blocks of this sequence are interspersed with other repetitive DNA sequences in this chromosome band. This study is the first to report a human repetitive DNA sequence uniquely localized to a specific chromosome. This clone provides an easily detected and highly specific chromosomal marker for molecular cytogenetic analyses in numerous basic research and clinical studies.

• Research Organization: Los Alamos National Lab., NM
• OSTI ID: 6791517
• Report Number(s): CONF-8606151-; TRN: 87-020136
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CHROMOSOMES
DNA SEQUENCING
DNA
HYBRIDIZATION
BIOLOGICAL LOCALIZATION
CELL FLOW SYSTEMS
CLONING
ELECTROPHORESIS
LABELLED COMPOUNDS
MAN
RADIOISOTOPES
RECOMBINANT DNA
TRACER TECHNIQUES
ANIMALS
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550401* - Genetics- Tracer Techniques