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Title: Diphtheria toxin can simultaneously bind to its receptor and adenylyl-(3',5')-uridine 3'-monophosphate

Abstract

Diphtheria toxin (DT) that was bound to receptors on BS-C-1 cells was able to bind approximately 1 molar equiv of adenylyl-(3',5')-uridine 3'-monophosphate (ApUp). In contrast, receptor-bound CRM197, a mutant form of toxin with greatly diminished affinity for dinucleotides, did not bind ApUp. Affinity of the dinucleotide for receptor-bound toxin differed from that for free toxin by less than an order of magnitude. These results indicate that the receptor site and the ApUp site on the toxin do not significantly overlap. BS-C-1 cells were incubated with or without /sup 125/I-DT or CRM 197. They were then incubated with (/sup 32/P)ApUp, and assayed.

Authors:
; ;
Publication Date:
Research Org.:
Harvard Medical School, Boston, MA
OSTI Identifier:
6790220
Alternate Identifier(s):
OSTI ID: 6790220
Resource Type:
Journal Article
Journal Name:
Biochemistry; (United States)
Additional Journal Information:
Journal Volume: 25:21
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; AMP; CONFIGURATION INTERACTION; TOXINS; RADIORECEPTOR ASSAY; UMP; ADENYLIC ACID; DIPHTHERIA; IODINE 125; PHOSPHORUS 32; RECEPTORS; ANTIGENS; BACTERIAL DISEASES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; DAYS LIVING RADIOISOTOPES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; INFECTIOUS DISEASES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; MATERIALS; MEMBRANE PROTEINS; NUCLEI; NUCLEOTIDES; ODD-EVEN NUCLEI; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PHOSPHORUS ISOTOPES; PROTEINS; RADIOISOTOPES; TOXIC MATERIALS; TRACER TECHNIQUES 550601* -- Medicine-- Unsealed Radionuclides in Diagnostics

Citation Formats

Barbieri, J.T., Collins, C.M., and Collier, R.J. Diphtheria toxin can simultaneously bind to its receptor and adenylyl-(3',5')-uridine 3'-monophosphate. United States: N. p., 1986. Web. doi:10.1021/bi00369a041.
Barbieri, J.T., Collins, C.M., & Collier, R.J. Diphtheria toxin can simultaneously bind to its receptor and adenylyl-(3',5')-uridine 3'-monophosphate. United States. doi:10.1021/bi00369a041.
Barbieri, J.T., Collins, C.M., and Collier, R.J. Tue . "Diphtheria toxin can simultaneously bind to its receptor and adenylyl-(3',5')-uridine 3'-monophosphate". United States. doi:10.1021/bi00369a041.
@article{osti_6790220,
title = {Diphtheria toxin can simultaneously bind to its receptor and adenylyl-(3',5')-uridine 3'-monophosphate},
author = {Barbieri, J.T. and Collins, C.M. and Collier, R.J.},
abstractNote = {Diphtheria toxin (DT) that was bound to receptors on BS-C-1 cells was able to bind approximately 1 molar equiv of adenylyl-(3',5')-uridine 3'-monophosphate (ApUp). In contrast, receptor-bound CRM197, a mutant form of toxin with greatly diminished affinity for dinucleotides, did not bind ApUp. Affinity of the dinucleotide for receptor-bound toxin differed from that for free toxin by less than an order of magnitude. These results indicate that the receptor site and the ApUp site on the toxin do not significantly overlap. BS-C-1 cells were incubated with or without /sup 125/I-DT or CRM 197. They were then incubated with (/sup 32/P)ApUp, and assayed.},
doi = {10.1021/bi00369a041},
journal = {Biochemistry; (United States)},
number = ,
volume = 25:21,
place = {United States},
year = {1986},
month = {10}
}