Intramitochondrial dyes allow selective in vitro photolysis of carcinoma cells
Carcinoma cell mitochondria preferentially accumulate and retain certain cationic dyes to a much greater extent than most normal cells. Thus, they can potentially serve as targets for highly selective photochemotherapy. We evaluated 10 rhodamine and cyanine dyes as carcinoma-specific mitochondrial photosensitizers in vitro. The most effective, N,N'-bis(2-ethyl-1,3-dioxolane)kryptocyanine (EDKC), caused marked, light-dependent killing of human bladder, squamous, and colon carcinoma cell lines after 30-min incubations at 1-0.01 microM but was minimally toxic to human keratinocytes and to normal monkey kidney epithelial cells (CV-1). Carcinoma cell phototoxicity was proportional to the amount of dye incorporated by the different cell lines. Selective killing ratios were 70-1000 for 0.1 microM dye and light doses of 100-175 J/cm2 between 680 and 720 nm.
- Research Organization:
- Harvard Medical School and Massachusetts General Hospital, Boston
- OSTI ID:
- 6777036
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 24
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DYES
RADIOSENSITIVITY EFFECTS
RHODAMINES
TUMOR CELLS
PHOTOLYSIS
CARCINOMAS
CELL KILLING
DOSE-RESPONSE RELATIONSHIPS
IN VITRO
MITOCHONDRIA
QUINOLINES
AMINES
ANIMAL CELLS
AROMATICS
AZAARENES
AZINES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMICAL REACTIONS
DECOMPOSITION
DISEASES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
NEOPLASMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANOIDS
PHOTOCHEMICAL REACTIONS
PYRIDINES
REAGENTS
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture