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Title: Bioremediation of PCB-contaminated soil at the Y-12 Plant

Abstract

The technical feasibility of bioremediation of soils contaminated with polychlorinated biphenyls (PCBs) was investigated using six in-situ lysimeters and two slurry bioreactors during the summer and fall of 1987. Microbial degradation of PCBs was characterized, and microbial cultures were isolated and characterized. Indigenous microorganisms present in contaminated soils from the floodplain of Bear Creek were shown to be capable of degrading monochlorinated biphenyl. Evidence included production of radiolabeled carbon dioxide from radiolabeled substrate and microbial characterization using gene probes and signature lipid analyses. Evidence was also obtained for dechlorination of highly chlorinated PCBs under laboratory conditions by microbial cultures isolated from other contaminated soils. These results are quite encouraging for further development of bioremediation technologies for PCBs. In-situ treatment of the soils in lysimeters by aeration/mixing and water appeared to stimulate growth of microorganisms and increase the biodegradation of monochlorinated biphenyl in laboratory experiments using soil samples from the lysimeters. The effects of additional nutrients, carbon source, and inocula were unclear from these tests; only one condition for each parameter was tested in the limited number of lysimeters. 12 refs., 11 figs., 14 tabs.

Authors:
; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Oak Ridge National Lab., TN (USA)
OSTI Identifier:
6770309
Report Number(s):
ORNL/TM-10750
ON: DE89001335
DOE Contract Number:  
AC05-84OR21400
Resource Type:
Technical Report
Resource Relation:
Other Information: Portions of this document are illegible in microfiche products
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; CHLORINATED ALIPHATIC HYDROCARBONS; BIODEGRADATION; LYSIMETERS; FIELD TESTS; SOILS; AERATION; MICROORGANISMS; REMEDIAL ACTION; TRACER TECHNIQUES; Y-12 PLANT; CHEMICAL REACTIONS; DECOMPOSITION; HALOGENATED ALIPHATIC HYDROCARBONS; ISOTOPE APPLICATIONS; MEASURING INSTRUMENTS; NATIONAL ORGANIZATIONS; ORGANIC CHLORINE COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; TESTING; US AEC; US DOE; US ERDA; US ORGANIZATIONS; 510200* - Environment, Terrestrial- Chemicals Monitoring & Transport- (-1989); 550700 - Microbiology

Citation Formats

Donaldson, T L, Strandberg, G W, McGinnis, G P, Palumbo, A V, White, D C, Hill, D L, Phelps, T J, Hadden, C T, Revis, N W, and Holdsworth, G. Bioremediation of PCB-contaminated soil at the Y-12 Plant. United States: N. p., 1988. Web.
Donaldson, T L, Strandberg, G W, McGinnis, G P, Palumbo, A V, White, D C, Hill, D L, Phelps, T J, Hadden, C T, Revis, N W, & Holdsworth, G. Bioremediation of PCB-contaminated soil at the Y-12 Plant. United States.
Donaldson, T L, Strandberg, G W, McGinnis, G P, Palumbo, A V, White, D C, Hill, D L, Phelps, T J, Hadden, C T, Revis, N W, and Holdsworth, G. Thu . "Bioremediation of PCB-contaminated soil at the Y-12 Plant". United States.
@article{osti_6770309,
title = {Bioremediation of PCB-contaminated soil at the Y-12 Plant},
author = {Donaldson, T L and Strandberg, G W and McGinnis, G P and Palumbo, A V and White, D C and Hill, D L and Phelps, T J and Hadden, C T and Revis, N W and Holdsworth, G},
abstractNote = {The technical feasibility of bioremediation of soils contaminated with polychlorinated biphenyls (PCBs) was investigated using six in-situ lysimeters and two slurry bioreactors during the summer and fall of 1987. Microbial degradation of PCBs was characterized, and microbial cultures were isolated and characterized. Indigenous microorganisms present in contaminated soils from the floodplain of Bear Creek were shown to be capable of degrading monochlorinated biphenyl. Evidence included production of radiolabeled carbon dioxide from radiolabeled substrate and microbial characterization using gene probes and signature lipid analyses. Evidence was also obtained for dechlorination of highly chlorinated PCBs under laboratory conditions by microbial cultures isolated from other contaminated soils. These results are quite encouraging for further development of bioremediation technologies for PCBs. In-situ treatment of the soils in lysimeters by aeration/mixing and water appeared to stimulate growth of microorganisms and increase the biodegradation of monochlorinated biphenyl in laboratory experiments using soil samples from the lysimeters. The effects of additional nutrients, carbon source, and inocula were unclear from these tests; only one condition for each parameter was tested in the limited number of lysimeters. 12 refs., 11 figs., 14 tabs.},
doi = {},
url = {https://www.osti.gov/biblio/6770309}, journal = {},
number = ,
volume = ,
place = {United States},
year = {1988},
month = {9}
}

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