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Title: Expression of smooth muscle and non-muscle myosin heavy chain isoforms in cultured vascular smooth muscle cells

Abstract

Immunocytochemical studies of cultured smooth muscle cells (SMCs) have disagreed on the nature of myosin expression. This investigation was undertaken to test for the presence of heterogeneous myosin heavy chain (MHC) isoforms in cell culture as a possible explanation for these results. Previously, Rovner et al. detected two MHCs in intact smooth muscles which differed in molecular weight by ca. 4000 daltons (SM1 and SM2) using a 3-4% acrylamide gradient SDS gel system. When sub-confluent primary cultures of rat aorta SMCs were assayed by this system, SM1 and SM2 were seen, along with large amounts of a third, unique MHC, NM, which closely resembled the MHC from human platelet in size and antigenicity. Data from /sup 35/S-methionine autoradiograms showed that the log growth phase SMC cultures were producing almost exclusively NM, but the growth arrest, post-confluent cultures synthesized increased relative amounts of the SM MHC forms and contained comparable amounts of SM1, SM2, and NM. The same patterns of MHC synthesis were seen in sub-passaged SMCs. The expression of the SM-specific forms of myosin in quiescent, post-confluent cultures parallels that of smooth muscle actin suggesting that density induced growth arrest promotes cytodifferentiation in cultured vascular SMCs.

Authors:
; ;
Publication Date:
Research Org.:
Univ. of Virginia School of Medicine, Charlottesville
OSTI Identifier:
6763784
Report Number(s):
CONF-8604222-
Journal ID: CODEN: FEPRA; TRN: 87-010351
Resource Type:
Journal Article
Journal Name:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
Additional Journal Information:
Journal Volume: 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; MYOSIN; MOLECULAR STRUCTURE; AUTORADIOGRAPHY; CELL CULTURES; METHIONINE; MUSCLES; SULFUR 35; AMINO ACIDS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBOXYLIC ACIDS; DAYS LIVING RADIOISOTOPES; DRUGS; EVEN-ODD NUCLEI; GLOBULINS; ISOTOPES; LIGHT NUCLEI; LIPOTROPIC FACTORS; NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; PROTEINS; RADIOISOTOPES; SULFUR ISOTOPES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Rovner, A S, Murphy, R A, and Owens, G K. Expression of smooth muscle and non-muscle myosin heavy chain isoforms in cultured vascular smooth muscle cells. United States: N. p., 1986. Web.
Rovner, A S, Murphy, R A, & Owens, G K. Expression of smooth muscle and non-muscle myosin heavy chain isoforms in cultured vascular smooth muscle cells. United States.
Rovner, A S, Murphy, R A, and Owens, G K. 1986. "Expression of smooth muscle and non-muscle myosin heavy chain isoforms in cultured vascular smooth muscle cells". United States.
@article{osti_6763784,
title = {Expression of smooth muscle and non-muscle myosin heavy chain isoforms in cultured vascular smooth muscle cells},
author = {Rovner, A S and Murphy, R A and Owens, G K},
abstractNote = {Immunocytochemical studies of cultured smooth muscle cells (SMCs) have disagreed on the nature of myosin expression. This investigation was undertaken to test for the presence of heterogeneous myosin heavy chain (MHC) isoforms in cell culture as a possible explanation for these results. Previously, Rovner et al. detected two MHCs in intact smooth muscles which differed in molecular weight by ca. 4000 daltons (SM1 and SM2) using a 3-4% acrylamide gradient SDS gel system. When sub-confluent primary cultures of rat aorta SMCs were assayed by this system, SM1 and SM2 were seen, along with large amounts of a third, unique MHC, NM, which closely resembled the MHC from human platelet in size and antigenicity. Data from /sup 35/S-methionine autoradiograms showed that the log growth phase SMC cultures were producing almost exclusively NM, but the growth arrest, post-confluent cultures synthesized increased relative amounts of the SM MHC forms and contained comparable amounts of SM1, SM2, and NM. The same patterns of MHC synthesis were seen in sub-passaged SMCs. The expression of the SM-specific forms of myosin in quiescent, post-confluent cultures parallels that of smooth muscle actin suggesting that density induced growth arrest promotes cytodifferentiation in cultured vascular SMCs.},
doi = {},
url = {https://www.osti.gov/biblio/6763784}, journal = {Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)},
number = ,
volume = 45:4,
place = {United States},
year = {Wed Mar 05 00:00:00 EST 1986},
month = {Wed Mar 05 00:00:00 EST 1986}
}