Effects of environmental conditions on xylose fermentation by recombinant Escherichia coli

Journal Article · Thu Feb 01 00:00:00 EST 1990 · Applied and Environmental Microbiology; (USA)

OSTI ID:6759628

Ohta, K; Alterthum, F; Ingram, L O ^[1]

---

1. Univ. of Florida, Gainesville (USA)

In batch fermentations, optimal conversion of xylose to ethanol by recombinant Escherichia coli was obtained under the following conditions: 30 to 37{degree}C, pH 6.4 to 6.8, 0.1 to 0.2 M potassium phosphate buffer, and xylose concentrations of 8% or less. A yield of 39.2 g of ethanol per liter (4.9% ethanol by volume) was observed with 80 g of xylose per liter, equivalent to 96% of the maximum theoretical yield. Maximal volumetric productivity was 0.7 g of ethanol per liter per h in batch fermentations and 30 g of ethanol per liter per h in concentrated cell suspensions (analogous to cell recycling).

• OSTI ID: 6759628
• Journal Information: Applied and Environmental Microbiology; (USA), Vol. 56:2; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
ESCHERICHIA COLI
GENETIC ENGINEERING
ETHANOL
BIOSYNTHESIS
XYLOSE
FERMENTATION
RECOMBINANT DNA
ALCOHOLS
ALDEHYDES
BACTERIA
BIOCONVERSION
CARBOHYDRATES
DNA
HYDROXY COMPOUNDS
MICROORGANISMS
MONOSACCHARIDES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PENTOSES
SACCHARIDES
SYNTHESIS
090900* - Biomass Fuels- Processing- (1990-)