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Title: Organization and control of genes encoding catabolic enzymes in Rhizobiaceae

Abstract

Rhizobiaceae, a diverse bacterial group comprising rhizobia and agrobacteria, symbiotic partnership with plants form nitrogen-fixing nodules on plant roots or are plant pathogens. Phenolic compounds produced by plants serve as inducers of rhizobial nodulation genes and agrobacterial virulence genes reflect their capacity to utilize numerous aromatics, including phenolics, as a source of carbon and energy. In many microbes the aerobic degradation of numerous aromatic compounds to tricarboxylic acid cycle intermediates is achieved by the [beta]-ketoadipate pathway. Our initial studies focused on the organization and regulation of the ketoadipate pathway in Agrobacterium tumefaciens. We have cloned, identified and characterized a novel regulatory gene that modulates expression of an adjacent pca (protocatechuate) structural gene, pcaD. Regulation of pcaD is mediated by the regulatory gene, termed pcaQ, in concert with the intermediate [beta]-carboxy-cis,cis-muconate. [beta]-carboxy-cis,cismuconate is an unstable chemical, not marketed commercially, and it is unlikely to permeate Escherichia coli cells if supplied in media. Because of these factors, characterization of pcaQ in E. coli required an in vivo delivery system for [beta]-carboxycis,cis-muconate. This was accomplished by designing an E. coli strain that expressed an Acinetobacter calcoaceticus pcaA gene for conversion of protocatechuate to [beta]-carboxy-cis,cis-muconate.

Authors:
;
Publication Date:
Research Org.:
Yale Univ., New Haven, CT (United States). Dept. of Biology
Sponsoring Org.:
DOE; USDOE, Washington, DC (United States)
OSTI Identifier:
6754773
Alternate Identifier(s):
OSTI ID: 6754773; Legacy ID: DE93010353
Report Number(s):
DOE/ER/13947-4
ON: DE93010353
DOE Contract Number:  
FG02-88ER13947
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; GENE OPERONS; DNA-CLONING; PHENOLS; BIODEGRADATION; RHIZOBIUM; MOLECULAR BIOLOGY; BIOLOGICAL PATHWAYS; CATABOLISM; GENE REGULATION; GENES; PROGRESS REPORT; AROMATICS; BACTERIA; CHEMICAL REACTIONS; CLONING; DECOMPOSITION; DNA HYBRIDIZATION; DOCUMENT TYPES; HYBRIDIZATION; HYDROXY COMPOUNDS; METABOLISM; MICROORGANISMS; ORGANIC COMPOUNDS 090900* -- Biomass Fuels-- Processing-- (1990-); 550200 -- Biochemistry; 550500 -- Metabolism; 550400 -- Genetics; 553000 -- Agriculture & Food Technology

Citation Formats

Parke, D., and Ornston, L.N.. Organization and control of genes encoding catabolic enzymes in Rhizobiaceae. United States: N. p., 1993. Web. doi:10.2172/6754773.
Parke, D., & Ornston, L.N.. Organization and control of genes encoding catabolic enzymes in Rhizobiaceae. United States. doi:10.2172/6754773.
Parke, D., and Ornston, L.N.. Mon . "Organization and control of genes encoding catabolic enzymes in Rhizobiaceae". United States. doi:10.2172/6754773. https://www.osti.gov/servlets/purl/6754773.
@article{osti_6754773,
title = {Organization and control of genes encoding catabolic enzymes in Rhizobiaceae},
author = {Parke, D. and Ornston, L.N.},
abstractNote = {Rhizobiaceae, a diverse bacterial group comprising rhizobia and agrobacteria, symbiotic partnership with plants form nitrogen-fixing nodules on plant roots or are plant pathogens. Phenolic compounds produced by plants serve as inducers of rhizobial nodulation genes and agrobacterial virulence genes reflect their capacity to utilize numerous aromatics, including phenolics, as a source of carbon and energy. In many microbes the aerobic degradation of numerous aromatic compounds to tricarboxylic acid cycle intermediates is achieved by the [beta]-ketoadipate pathway. Our initial studies focused on the organization and regulation of the ketoadipate pathway in Agrobacterium tumefaciens. We have cloned, identified and characterized a novel regulatory gene that modulates expression of an adjacent pca (protocatechuate) structural gene, pcaD. Regulation of pcaD is mediated by the regulatory gene, termed pcaQ, in concert with the intermediate [beta]-carboxy-cis,cis-muconate. [beta]-carboxy-cis,cismuconate is an unstable chemical, not marketed commercially, and it is unlikely to permeate Escherichia coli cells if supplied in media. Because of these factors, characterization of pcaQ in E. coli required an in vivo delivery system for [beta]-carboxycis,cis-muconate. This was accomplished by designing an E. coli strain that expressed an Acinetobacter calcoaceticus pcaA gene for conversion of protocatechuate to [beta]-carboxy-cis,cis-muconate.},
doi = {10.2172/6754773},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Mar 01 00:00:00 EST 1993},
month = {Mon Mar 01 00:00:00 EST 1993}
}

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