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Title: Biomarker responses in cyprinids of the middle stretch of the River Po, Italy

Abstract

Fish belonging to three species of cyprinids, that is, barbel (Barbus plebejus), chub (Leuciscus cephalus), and Italian nase (Chondrostoma soeetta), were collected from two sites of the River Po, located upstream and downstream from the confluence of one of its middle-reach polluted tributaries, the River Lambro. The two groups of individuals caught for each species were analyzed and compared for several microsomal and cytosolic biochemical markers. The enzymatic activities assayed in fish liver included ethoxyresorufin O-deethylase (EROD), aminopyrine-N-demethylase (APDM), uridine diphosphate glucuronyltransferase (UDPGT), glutathione S-transferase (GST), glutathione reductase, and glutathione peroxidase. In addition, the contents of reduced glutathione and nonprotein thiols were measured. Despite some differences among species, all microsomal activities (EROD, APDM, UDPGT) were found to be significantly induced in fish living downstream the River Lambro. With the exception of a higher GST enzyme activity of barbel from the downstream reach, no significant modification was evident in any of the tested cytosolic biomarkers. Results showed that barbel and nase better discriminated the two reaches of the River Po. In general, the alterations observed in feral fish are consistent with the results found in previous studies conducted with rainbow trout (Oncorhynchus mykiss) under both laboratory and field conditions in themore » same middle reach of the River Po. All of the data indicate that the downstream tract of the main river is exposed to the load of pollutants transported by the River Lambro, including known inducers such as polychlorinated biphenyls and polycyclic aromatic hydrocarbons (PAHs). The latter were analyzed in sediments sampled at the two sites of fish collection, and the downstream sediment showed the highest concentrations of PAHs, although their levels are comparable to those present in moderately polluted locations. Regardless of the site of exposure, barbel seem to be characterized by more efficient antioxidant defenses.« less

Authors:
 [1]; ;  [2]; ;  [3]
  1. National Council of Research, Milan (Italy). Water Research Inst.
  2. Univ. of Genoa (Italy). Inst. of Zoology
  3. Presidio Multizonale Igiene Prevenzione, Lecco (Italy)
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
675456
Resource Type:
Journal Article
Journal Name:
Environmental Toxicology and Chemistry
Additional Journal Information:
Journal Volume: 17; Journal Issue: 3; Other Information: PBD: Mar 1998
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 56 BIOLOGY AND MEDICINE, APPLIED STUDIES; WATER POLLUTION; ITALY; RIVERS; BIOLOGICAL INDICATORS; BIOLOGICAL MARKERS; POLYCHLORINATED BIPHENYLS; POLYCYCLIC AROMATIC HYDROCARBONS; FISHES

Citation Formats

Vigano, L, Arillo, A, Melodia, F, Arlati, P, and Monti, C. Biomarker responses in cyprinids of the middle stretch of the River Po, Italy. United States: N. p., 1998. Web. doi:10.1002/etc.5620170309.
Vigano, L, Arillo, A, Melodia, F, Arlati, P, & Monti, C. Biomarker responses in cyprinids of the middle stretch of the River Po, Italy. United States. https://doi.org/10.1002/etc.5620170309
Vigano, L, Arillo, A, Melodia, F, Arlati, P, and Monti, C. Sun . "Biomarker responses in cyprinids of the middle stretch of the River Po, Italy". United States. https://doi.org/10.1002/etc.5620170309.
@article{osti_675456,
title = {Biomarker responses in cyprinids of the middle stretch of the River Po, Italy},
author = {Vigano, L and Arillo, A and Melodia, F and Arlati, P and Monti, C},
abstractNote = {Fish belonging to three species of cyprinids, that is, barbel (Barbus plebejus), chub (Leuciscus cephalus), and Italian nase (Chondrostoma soeetta), were collected from two sites of the River Po, located upstream and downstream from the confluence of one of its middle-reach polluted tributaries, the River Lambro. The two groups of individuals caught for each species were analyzed and compared for several microsomal and cytosolic biochemical markers. The enzymatic activities assayed in fish liver included ethoxyresorufin O-deethylase (EROD), aminopyrine-N-demethylase (APDM), uridine diphosphate glucuronyltransferase (UDPGT), glutathione S-transferase (GST), glutathione reductase, and glutathione peroxidase. In addition, the contents of reduced glutathione and nonprotein thiols were measured. Despite some differences among species, all microsomal activities (EROD, APDM, UDPGT) were found to be significantly induced in fish living downstream the River Lambro. With the exception of a higher GST enzyme activity of barbel from the downstream reach, no significant modification was evident in any of the tested cytosolic biomarkers. Results showed that barbel and nase better discriminated the two reaches of the River Po. In general, the alterations observed in feral fish are consistent with the results found in previous studies conducted with rainbow trout (Oncorhynchus mykiss) under both laboratory and field conditions in the same middle reach of the River Po. All of the data indicate that the downstream tract of the main river is exposed to the load of pollutants transported by the River Lambro, including known inducers such as polychlorinated biphenyls and polycyclic aromatic hydrocarbons (PAHs). The latter were analyzed in sediments sampled at the two sites of fish collection, and the downstream sediment showed the highest concentrations of PAHs, although their levels are comparable to those present in moderately polluted locations. Regardless of the site of exposure, barbel seem to be characterized by more efficient antioxidant defenses.},
doi = {10.1002/etc.5620170309},
url = {https://www.osti.gov/biblio/675456}, journal = {Environmental Toxicology and Chemistry},
number = 3,
volume = 17,
place = {United States},
year = {1998},
month = {3}
}