skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Human sperm chromosomes. Long-term effect of cancer treatment

Abstract

The long-term cytogenetic effect of radio- or chemotherapy or both on male germ cells was evaluated by study of the chromosomal abnormalities in spermatozoa of four men treated for cancer 5-18 years earlier. The cytogenetic analysis of 422 sperm metaphases showed no differences in the aneuploidy rate. The incidence of structural chromosome aberrations was 14.0%, however, which is much higher than in controls. Thus, the high incidence of structurally aberrant spermatozoa observed in our long-term study indicates that antitumoral treatments affect stem-cell spermatogonia and that aberrant cells can survive germinal selection and produce abnormal spermatozoa.

Authors:
; ; ; ; ;  [1]
  1. (Universitat Autonoma de Barcelona (Spain))
Publication Date:
OSTI Identifier:
6748895
Resource Type:
Journal Article
Resource Relation:
Journal Name: Cancer Genetics and Cytogenetics; (USA); Journal Volume: 46:2
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CHROMOSOMAL ABERRATIONS; RADIOINDUCTION; RADIOTHERAPY; SIDE EFFECTS; SPERMATOZOA; BIOLOGICAL RADIATION EFFECTS; ANTINEOPLASTIC DRUGS; KARYOTYPE; RADIATION INJURIES; BIOLOGICAL EFFECTS; DRUGS; GAMETES; GERM CELLS; INJURIES; MEDICINE; MUTATIONS; NUCLEAR MEDICINE; RADIATION EFFECTS; RADIOLOGY; THERAPY 560151* -- Radiation Effects on Animals-- Man

Citation Formats

Genesca, A., Caballin, M.R., Miro, R., Benet, J., Bonfill, X., and Egozcue, J.. Human sperm chromosomes. Long-term effect of cancer treatment. United States: N. p., 1990. Web.
Genesca, A., Caballin, M.R., Miro, R., Benet, J., Bonfill, X., & Egozcue, J.. Human sperm chromosomes. Long-term effect of cancer treatment. United States.
Genesca, A., Caballin, M.R., Miro, R., Benet, J., Bonfill, X., and Egozcue, J.. 1990. "Human sperm chromosomes. Long-term effect of cancer treatment". United States. doi:.
@article{osti_6748895,
title = {Human sperm chromosomes. Long-term effect of cancer treatment},
author = {Genesca, A. and Caballin, M.R. and Miro, R. and Benet, J. and Bonfill, X. and Egozcue, J.},
abstractNote = {The long-term cytogenetic effect of radio- or chemotherapy or both on male germ cells was evaluated by study of the chromosomal abnormalities in spermatozoa of four men treated for cancer 5-18 years earlier. The cytogenetic analysis of 422 sperm metaphases showed no differences in the aneuploidy rate. The incidence of structural chromosome aberrations was 14.0%, however, which is much higher than in controls. Thus, the high incidence of structurally aberrant spermatozoa observed in our long-term study indicates that antitumoral treatments affect stem-cell spermatogonia and that aberrant cells can survive germinal selection and produce abnormal spermatozoa.},
doi = {},
journal = {Cancer Genetics and Cytogenetics; (USA)},
number = ,
volume = 46:2,
place = {United States},
year = 1990,
month = 6
}
  • Fluorescence in situ hybridization with repetitive-sequence DNA probes was used to detect human sperm disomic for chromosomes 1 and Y in three healthy men. Data on these same men had been obtained previously, using the human-sperm/hamster-egg cytogenetic technique, providing a cytogenetic reference for validating sperm hybridization measurements. Air-dried smears were prepared from semen samples and treated with DTT and lithium diiodosalicylate to expand sperm chromatin. Hybridization with fluorescently tagged DNA probes for chromosomes 1 (pUC177) or Y (pY3.4) yielded average frequencies of sperm with two fluorescent domains of 14.2[+-]2.4/10,000 and 5.6[+-]1.6/10,000 sperm, respectively. These frequencies did not differ statistically frommore » frequencies of hyperploidy observed for these chromosomes with the hamster technique. In addition, frequencies of disomic sperm from one donor were elevated [approximately]2.5-fold above those of other donors, for both chromosomes 1 (P = .045) and Y (P = .01), consistent with a trend found with the hamster technique. The authors conclude that fluorescence in situ hybridization to sperm chromosomes provides a valid and promising measure of the frequency of disomic human sperm. 43 refs., 1 fig., 4 tabs.« less
  • Human sperm chromosomes were obtained after capacitation with TES-Tris (TEST) yolk buffer and fusion with Syrian hamster eggs. Semen samples could be stored at 4/sup 0/C for 3 days and remain functional in the assay system. The efficiency of TEST yolk buffer for obtaining karyotypes was as good as, or greater than, the efficiency of standard BWW medium containing human serum albumin. 16 references, 3 tables.
  • Purpose: To evaluate the long-term health-related quality of life (HRQL) after external beam radiation therapy (EBRT) or vaginal brachytherapy (VBT) among PORTEC-2 trial patients, evaluate long-term bowel and bladder symptoms, and assess the impact of cancer on these endometrial cancer (EC) survivors. Patients and Methods: In the PORTEC-2 trial, 427 patients with stage I high–intermediate-risk EC were randomly allocated to EBRT or VBT. The 7- and 10-year HRQL questionnaires consisted of EORTC QLQ-C30; subscales for bowel and bladder symptoms; the Impact of Cancer Questionnaire; and 14 questions on comorbidities, walking aids, and incontinence pads. Analysis was done using linear mixed modelsmore » for subscales and (ordinal) logistic regression with random effects for single items. A two-sided P value <.01 was considered statistically significant. Results: Longitudinal HRQL analysis showed persisting higher rates of bowel symptoms with EBRT, without significant differences in global health or any of the functioning scales. At 7 years, clinically relevant fecal leakage was reported by 10.6% in the EBRT group, versus 1.8% for VBT (P=.03), diarrhea by 8.4% versus 0.9% (P=.04), limitations due to bowel symptoms by 10.5% versus 1.8% (P=.001), and bowel urgency by 23.3% versus 6.6% (P<.001). Urinary urgency was reported by 39.3% of EBRT patients, 25.5% for VBT, P=.05. No difference in sexual activity was seen between treatment arms. Long-term impact of cancer scores was higher among the patients who had an EC recurrence or second cancer. Conclusions: More than 7 years after treatment, EBRT patients reported more bowel symptoms with impact on daily activities, and a trend for more urinary symptoms, without impact on overall quality of life or difference in cancer survivorship issues.« less