Characterization of a major 31-kilodalton peptidoglycan-bound protein of Legionella pneumophila

Journal Article · Tue May 01 00:00:00 EDT 1990 · Journal of Bacteriology; (USA)

OSTI ID:6746885

Butler, C A; Hoffman, P S ^[1]

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1. Univ. of Tennessee, Memphis (USA)

A 31-kilodalton (kDa) protein was solubilized from the peptidoglycan (PG) fraction of Legionella pneumophila after treatment with either N-acetylmuramidase from the fungus Chalaropsis sp. or with mutanolysin from Streptomyces globisporus. The protein exhibited a ladderlike banding pattern by autoradiography when radiolabeled ((35S)cysteine or (35S)methionine) PG material was extensively treated with hen lysozyme. The banding patterns ranging between 31 and 45 kDa and between 55 and 60 kDa resolved as a single 31-kDa protein when the material was subsequently treated with N-acetylmuramidase. Analysis of the purified 31-kDa protein for diaminopimelic acid by gas chromatography revealed 1 mol of diaminopimelic acid per mol of protein. When outer membrane PG material containing the major outer membrane porin protein was treated with N-acetylmuramidase or mutanolysin, both the 28.5-kDa major outer membrane protein and the 31-kDa protein were solubilized from the PG material under reducing conditions. In the absence of 2-mercaptoethanol, a high-molecular-mass complex (100 kDa) was resolved. The results of this study indicate that a 31-kDa PG-bound protein is a major component of the cell wall of L. pneumophila whose function may be to anchor the major outer membrane protein to PG. Finally, a survey of other Legionella species and other serogroups of L. pneumophila suggested that PG-bound proteins may be a common feature of this genus.

• OSTI ID: 6746885
• Journal Information: Journal of Bacteriology; (USA), Vol. 172:5; ISSN 0021-9193
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLYCOPROTEINS
CHEMICAL COMPOSITION
LEGIONELLA PNEUMOPHILA
BIOCHEMISTRY
AUTORADIOGRAPHY
CYSTEINE
ELECTROPHORESIS
GLYCOSYL HYDROLASES
METHIONINE
MOLECULAR WEIGHT
PORINS
SULFUR 35
AMINO ACIDS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DRUGS
ENZYMES
EVEN-ODD NUCLEI
HYDROLASES
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MEMBRANE PROTEINS
MICROORGANISMS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PROTEINS
RADIOISOTOPES
SULFUR ISOTOPES
THIOLS
550201* - Biochemistry- Tracer Techniques