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Title: Localization of human immunodeficiency virus antigens in infected cells by scanning/transmission-immunogold techniques

Abstract

An application of high resolution scanning/transmission electron microscopy (STEM) and gold-labelling techniques for the rapid detection of human immunodeficiency virus (HIV) in infected cells has been developed. Experimental in vitro studies for detecting two HIV structural proteins, gp41 and p17, were performed following an indirect labeling procedure that uses monoclonal anti-p17 and anti-gp41 antibodies as primary antibodies and 40 nm gold-linked goat antimouse IgG as secondary antibodies. The cells were then studied by STEM in the scanning mode. Unambiguous localization of the viral antigens was possible by combining the three-dimensional image provided by the secondary electron image and the atomic number-dependent backscattered electron image for the identification of the gold marker. This technique combines both the morphological information and the rapid procedures of scanning electron microscopy with the precise and sensitive antigen detection provided by the use of STEM and immunological methods. The preliminary results of its application to the study of peripheral blood mononuclear cells from four anti-HIV-seropositive patients showing the presence of specific labeling in all of them suggest that it might prove useful for early detection of HIV infection before seroconversion, as well as for quantitative studies.

Authors:
; ; ;
Publication Date:
Research Org.:
Centro Nacional de Microbiologia, Madrid (Spain)
OSTI Identifier:
6745866
Resource Type:
Journal Article
Journal Name:
Ultrastruct. Pathol.; (United States)
Additional Journal Information:
Journal Volume: 12:4
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; 59 BASIC BIOLOGICAL SCIENCES; AIDS VIRUS; ANTIGENS; BIOLOGICAL LOCALIZATION; AIDS; GOLD ISOTOPES; IN VITRO; LYMPHOCYTES; MONOCLONAL ANTIBODIES; SCANNING ELECTRON MICROSCOPY; TRACER TECHNIQUES; ANIMAL CELLS; ANTIBODIES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CONNECTIVE TISSUE CELLS; DISEASES; ELECTRON MICROSCOPY; HEMIC DISEASES; IMMUNE SYSTEM DISEASES; INFECTIOUS DISEASES; ISOTOPE APPLICATIONS; ISOTOPES; LEUKOCYTES; MATERIALS; MICROORGANISMS; MICROSCOPY; PARASITES; SOMATIC CELLS; VIRAL DISEASES; VIRUSES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics; 550901 - Pathology- Tracer Techniques

Citation Formats

Herrera, M I, Santa Maria, I, de Andres, R, and Najera, R. Localization of human immunodeficiency virus antigens in infected cells by scanning/transmission-immunogold techniques. United States: N. p., 1988. Web. doi:10.3109/01913128809064213.
Herrera, M I, Santa Maria, I, de Andres, R, & Najera, R. Localization of human immunodeficiency virus antigens in infected cells by scanning/transmission-immunogold techniques. United States. https://doi.org/10.3109/01913128809064213
Herrera, M I, Santa Maria, I, de Andres, R, and Najera, R. 1988. "Localization of human immunodeficiency virus antigens in infected cells by scanning/transmission-immunogold techniques". United States. https://doi.org/10.3109/01913128809064213.
@article{osti_6745866,
title = {Localization of human immunodeficiency virus antigens in infected cells by scanning/transmission-immunogold techniques},
author = {Herrera, M I and Santa Maria, I and de Andres, R and Najera, R},
abstractNote = {An application of high resolution scanning/transmission electron microscopy (STEM) and gold-labelling techniques for the rapid detection of human immunodeficiency virus (HIV) in infected cells has been developed. Experimental in vitro studies for detecting two HIV structural proteins, gp41 and p17, were performed following an indirect labeling procedure that uses monoclonal anti-p17 and anti-gp41 antibodies as primary antibodies and 40 nm gold-linked goat antimouse IgG as secondary antibodies. The cells were then studied by STEM in the scanning mode. Unambiguous localization of the viral antigens was possible by combining the three-dimensional image provided by the secondary electron image and the atomic number-dependent backscattered electron image for the identification of the gold marker. This technique combines both the morphological information and the rapid procedures of scanning electron microscopy with the precise and sensitive antigen detection provided by the use of STEM and immunological methods. The preliminary results of its application to the study of peripheral blood mononuclear cells from four anti-HIV-seropositive patients showing the presence of specific labeling in all of them suggest that it might prove useful for early detection of HIV infection before seroconversion, as well as for quantitative studies.},
doi = {10.3109/01913128809064213},
url = {https://www.osti.gov/biblio/6745866}, journal = {Ultrastruct. Pathol.; (United States)},
number = ,
volume = 12:4,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1988},
month = {Fri Jan 01 00:00:00 EST 1988}
}