Effect of in vitro and in vivo UV irradiation on the production of ETAF activity by human and murine keratinocytes

Journal Article · Thu Dec 01 00:00:00 EST 1983 · J. Invest. Dermatol.; (United States)

DOI:https://doi.org/10.1111/1523-1747.ep12522862· OSTI ID:6743130

Ansel, J C; Luger, T A; Green, I

Cultured epidermal cells and keratinocytes produce a potent hormone-like factor called epidermal cell-derived thymocyte-activating factor (ETAF). ETAF appears to be similar if not identical to a monocyte-derived lymphokine, known as interleukin 1 (IL-1). These two cytokines are able to amplify a diverse number of proliferative and inflammatory processes. Several recent investigations have suggested that UV-induced immunosuppression may be due in part to the inhibition of IL-1/ETAF production by monocytes and keratinocytes, respectively. We therefore decided to directly study the effects of various doses of in vitro and in vivo UV radiation (UVR) on the production of ETAF by normal murine epidermal cells and a murine (Pam 212) and a human (SCC) keratinocyte cell line. Our results surprisingly demonstrated an increase in both the extracellular and the intracellular ETAF activity of the murine epidermal, Pam 212, and SCC after sublethal amounts of in vitro UVR. Likewise, increased ETAF activity of murine epidermal cells was detected after sublethal doses of in vivo UVR. The UV-induced ETAF activity was cycloheximide-sensitive, suggesting that de novo synthesis of ETAF rather than cell membrane leakage was responsible for the increased ETAF activity. The fact that UV irradiation can increase ETAF activity by keratinocytes could have important local and systemic consequences for the host and may provide an efficient, contaminant-free method for generating ETAF activity for further biochemical and immunologic studies.

• Research Organization: National Inst. of Allergy and Infectious Diseases, Bethesda, MD
• OSTI ID: 6743130
• Journal Information: J. Invest. Dermatol.; (United States), Vol. 81:6
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL RADIATION EFFECTS
HORMONES
BIOSYNTHESIS
INHIBITION
CELL CULTURES
CELL PROLIFERATION
IMMUNOSUPPRESSION
INFLAMMATION
MAN
MICE
SKIN
ULTRAVIOLET RADIATION
ANIMALS
BIOLOGICAL EFFECTS
BODY
ELECTROMAGNETIC RADIATION
MAMMALS
ORGANS
PATHOLOGICAL CHANGES
PRIMATES
RADIATION EFFECTS
RADIATIONS
RODENTS
SYMPTOMS
SYNTHESIS
VERTEBRATES
560121* - Radiation Effects on Cells- External Source- (-1987)