Genetic control of murine T cell proliferative responses to Mycobacterium leprae. V. Evidence for cross-reactivity between host antigens and Mycobacterium leprae
T cell proliferative responses to Mycobacterium leprae were measured by immunization of mice at the base of the tail with Ag and challenging lymphocytes from draining lymph nodes in culture with M. leprae. C57BL/10J and B10.BR mice were identified as low responder mice and the congenic strains B10.M, B10.Q, and B10.AKM as high responders whereas F1 (high x low) hybrid mice were found to be low responders. The cellular basis of low responsiveness did not appear to result from a defect in Ag-presenting cells or the activation of suppressor T cells by M. leprae. The influence of the environment in which T cells developed on responsiveness to M. leprae was analyzed in chimeric mice prepared by irradiating F1(C57BL/10J x B10.M) mice and reconstituting with bone marrow from C57BL/10J, B10.M, or F1 donors. Six weeks later, chimeric mice were immunized with M. leprae, lymph node cells were subsequently prepared, and H-2 phenotyped and challenged in culture with M. leprae Ag. T cell proliferative responses were found to be low in all cases, similar to those observed using lymph node cells from F1 hybrid mice. These results suggested that high responder B10.M lymphocytes developing in the irradiated F1 mice became tolerized to antigenic determinants found on M. leprae. This implied cross-reactive epitopes existed between some mouse strains and M. leprae. Low responsiveness to M. leprae in low responder and F1 hybrid mice may result from tolerance to H-2-encoded Ag that show cross-reactivity with M. leprae.
- Research Organization:
- Univ. of Auckland (New Zealand)
- OSTI ID:
- 6729366
- Journal Information:
- J. Immunol.; (United States), Vol. 141:5
- Country of Publication:
- United States
- Language:
- English
Similar Records
Clonal deletion: A mechanism of tolerance in mixed bone marrow chimeras
Leucyl-leucine methyl ester treatment of donor cells permits establishment of immunocompetent parent----F1 chimeras that are selectively tolerant of host alloantigens
Related Subjects
IMMUNITY
RADIOINDUCTION
LYMPHOCYTES
CELL PROLIFERATION
MYCOBACTERIUM
INFECTIVITY
ANTIGENS
BONE MARROW
LEPROSY
LYMPH NODES
MICE
PHENOTYPE
RADIATION CHIMERAS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BACTERIA
BACTERIAL DISEASES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CHIMERAS
CONNECTIVE TISSUE CELLS
DISEASES
HEMATOPOIETIC SYSTEM
INFECTIOUS DISEASES
LEUKOCYTES
LYMPHATIC SYSTEM
MAMMALS
MATERIALS
MICROORGANISMS
MOSAICISM
ORGANS
RODENTS
SOMATIC CELLS
TISSUES
VERTEBRATES
560152* - Radiation Effects on Animals- Animals