skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Some nutritional effects of folate-binding protein in bovine milk on the bioavailability of folate to rats

Abstract

The excretions of folate compounds into both the urine and bile were investigated in rats after the administration of pteroylglutamic acid (PteGlu) with or without the folate-binding protein (FBP) prepared from bovine milk. When the sample solution, containing either free or bound (/sup 3/H)PteGlu (i.e., bound to the FBP from milk), was delivered to rats intragastrically via oral intubation, the amounts of (/sup 3/H)PteGlu excreted into the feces did not change. On the other hand, the urinary excretion of /sup 3/H-labeled folate compounds, especially (/sup 3/H)5-methyltetrahydrofolic acid (5-CH/sub 3/-H/sub 4/PteGlu), after the administration of bound (/sup 3/H)PteGlu was significantly lower (P less than 0.01) than that after the administration of free (/sup 3/H)PteGlu. The urinary excretion of (/sup 3/H)5-CH/sub 3/-H/sub 4/PteGlu was directly proportional to the initial amount of free (/sup 3/H)PteGlu administered. The similar effect of FBP was also observed when the biliary excretion of /sup 3/H-labeled folate compounds was investigated in situ. Furthermore, the incorporation of (/sup 3/H)PteGlu into folate-requiring intestinal microorganisms was considerably reduced when it was bound to FBP. These results suggest that milk FBP has some nutritional effects on the bioavailability of folate in vivo.

Authors:
;
Publication Date:
Research Org.:
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Japan
OSTI Identifier:
6728641
Resource Type:
Journal Article
Journal Name:
J. Nutr.; (United States)
Additional Journal Information:
Journal Volume: 114:4
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; FOLIC ACID; EXCRETION; METABOLISM; CARBON 14 COMPOUNDS; DIET; LIQUID COLUMN CHROMATOGRAPHY; MILK; RATS; TRACER TECHNIQUES; TRITIUM COMPOUNDS; AMINO ACIDS; ANIMALS; AROMATICS; AZAARENES; BIOLOGICAL MATERIALS; BODY FLUIDS; CARBOXYLIC ACIDS; CHROMATOGRAPHY; CLEARANCE; DRUGS; FOOD; HEMATINICS; HEMATOLOGIC AGENTS; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MAMMALS; MATERIALS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PTERIDINES; RODENTS; SEPARATION PROCESSES; VERTEBRATES; VITAMIN B GROUP; VITAMINS; 550501* - Metabolism- Tracer Techniques

Citation Formats

Tani, M, and Iwai, K. Some nutritional effects of folate-binding protein in bovine milk on the bioavailability of folate to rats. United States: N. p., 1984. Web.
Tani, M, & Iwai, K. Some nutritional effects of folate-binding protein in bovine milk on the bioavailability of folate to rats. United States.
Tani, M, and Iwai, K. 1984. "Some nutritional effects of folate-binding protein in bovine milk on the bioavailability of folate to rats". United States.
@article{osti_6728641,
title = {Some nutritional effects of folate-binding protein in bovine milk on the bioavailability of folate to rats},
author = {Tani, M and Iwai, K},
abstractNote = {The excretions of folate compounds into both the urine and bile were investigated in rats after the administration of pteroylglutamic acid (PteGlu) with or without the folate-binding protein (FBP) prepared from bovine milk. When the sample solution, containing either free or bound (/sup 3/H)PteGlu (i.e., bound to the FBP from milk), was delivered to rats intragastrically via oral intubation, the amounts of (/sup 3/H)PteGlu excreted into the feces did not change. On the other hand, the urinary excretion of /sup 3/H-labeled folate compounds, especially (/sup 3/H)5-methyltetrahydrofolic acid (5-CH/sub 3/-H/sub 4/PteGlu), after the administration of bound (/sup 3/H)PteGlu was significantly lower (P less than 0.01) than that after the administration of free (/sup 3/H)PteGlu. The urinary excretion of (/sup 3/H)5-CH/sub 3/-H/sub 4/PteGlu was directly proportional to the initial amount of free (/sup 3/H)PteGlu administered. The similar effect of FBP was also observed when the biliary excretion of /sup 3/H-labeled folate compounds was investigated in situ. Furthermore, the incorporation of (/sup 3/H)PteGlu into folate-requiring intestinal microorganisms was considerably reduced when it was bound to FBP. These results suggest that milk FBP has some nutritional effects on the bioavailability of folate in vivo.},
doi = {},
url = {https://www.osti.gov/biblio/6728641}, journal = {J. Nutr.; (United States)},
number = ,
volume = 114:4,
place = {United States},
year = {Sun Apr 01 00:00:00 EST 1984},
month = {Sun Apr 01 00:00:00 EST 1984}
}