Enzymatic synthesis of formic acid from H/sub 2/ and CO/sub 2/ and production of hydrogen from formic acid. [Alcaligenes eutrophus]
Whole cells of Alcaligenes eutrophus (as well as isolated P. oxalaticus formate dehydrogenase and A. eutrophus hydrogenase coupled via NAD+ or methyl viologen) have been shown to produce H/sub 2/ from formic acid. Immobilization of the cells in Kappa-carrageenan gel greatly enhances their stability at room temperature. The rate of hydrogen production catalyzed by immobilized A. eutrophus has been studied as a function of the concentrations of the cells and formate and also pH. An inhibition by high concentrations of formate has been found. Immobilized cells were also capable of synthesizing formate from H/sub 2/ and bicarbonate. Yields of formate up to 30% have been obtained. The catalytic efficiency of immobilized A. eutrophus cells was compared with that of palladium adsorbed on activated carbon. (Refs. 35).
- Research Organization:
- Lab Applied Biochemistry, Dept Nutrition and Food Science, Massachusetts Inst Tech Cambridge, MA 02139
- OSTI ID:
- 6723418
- Journal Information:
- Biotechnol. Bioeng.; (United States), Vol. 24:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
09 BIOMASS FUELS
BACTERIA
IMMOBILIZED CELLS
FORMIC ACID
BIOSYNTHESIS
HYDROGEN PRODUCTION
CARBON DIOXIDE
CATALYTIC EFFECTS
CHEMICAL FEEDSTOCKS
ENZYMES
SUBSTRATES
CARBON COMPOUNDS
CARBON OXIDES
CARBOXYLIC ACIDS
CHALCOGENIDES
MICROORGANISMS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
SYNTHESIS
080106* - Hydrogen- Production- Biosynthesis & Photochemical Processes
550700 - Microbiology
140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989)