Trapping of DNA-reactive metabolites of therapeutic or carcinogenic agents by /sup 14/C-labeled synthetic polynucleotides

Journal Article · Sun Aug 01 00:00:00 EDT 1982 · Cancer Res.; (United States)

OSTI ID:6711875

Mehta, J R; Ludlum, D B

Many substances which do not react with DNA directly are metabolized into important DNA-modifying intermediates. We have devised a method for trapping these intermediates with /sup 14/C-labeled nucleosides contained in a synthetic polynucleotide. The polynucleotide structure protects the labeled nucleoside from metabolism; thus, it is unaltered when the polymer is incubated with a drug-metabolizing system. However, when the polymer is incubated with this system and a compound which can be metabolized into a reactive species, these intermediates are trapped by the /sup 14/C-labeled nucleoside and subsequently are detected as new peaks of radioactivity in a digest of the labeled polynucleotide. This system has been used to detect reactive intermediates of cyclophosphamide generated by a liver homogenate.

• Research Organization: Department of Medicine, Albany Medical College, Albany, New York
• OSTI ID: 6711875
• Journal Information: Cancer Res.; (United States), Vol. 42:8
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CARBON 14 COMPOUNDS
ISOTOPE APPLICATIONS
ENDOXAN
BIOCHEMICAL REACTION KINETICS
METABOLISM
DNA
METABOLITES
NUCLEOTIDES
ALKYLATING AGENTS
DRUGS
IMMUNOSUPPRESSIVE DRUGS
KINETICS
LABELLED COMPOUNDS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
REACTION KINETICS
550201* - Biochemistry- Tracer Techniques