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Title: Quantitative reverse sample genome probing of microbial communities and its application to oil field production waters

Abstract

This paper presents a protocol for quantitative analysis of microbial communities by reverse sample genome probing is presented in which (i) whole community DNA is isolated and labeled in the presence of a known amount of an added internal standard and (ii) the resulting spiked reverse genome probe is hybridized with a master filter on which denatured genomic DNAs from bacterial standards isolated from the target environment were spotted in large amounts (up to 1,500 ng) in order to improve detection sensitivity. This protocol allowed reproducible fingerprinting of the microbial community in oil field production waters at 19 sites from which water and biofilm samples were collected. It appeared that selected sulfate-reducing bacteria were significantly enhanced in biofilms covering the metal surfaces in contact with the production waters.

Authors:
; ; ;  [1];  [2];  [3]
  1. Univ. of Calgary, Alberta (Canada)
  2. Novacor Research and Technology Corporation, Calgary, Alberta (Canada)
  3. Univ. of Alberta (Canada)
Publication Date:
OSTI Identifier:
6703677
Resource Type:
Journal Article
Journal Name:
Applied and Environmental Microbiology; (United States)
Additional Journal Information:
Journal Volume: 59:12; Journal ID: ISSN 0099-2240
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 02 PETROLEUM; DNA; EVALUATION; MICROORGANISMS; GENES; OIL FIELDS; PATTERN RECOGNITION; PROBES; GEOLOGIC DEPOSITS; MINERAL RESOURCES; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PETROLEUM DEPOSITS; RESOURCES; 560300* - Chemicals Metabolism & Toxicology; 020000 - Petroleum

Citation Formats

Voordouw, G, Shen, Y, Harrington, C S, Teland, A J, Jack, T R, and Westlake, W S. Quantitative reverse sample genome probing of microbial communities and its application to oil field production waters. United States: N. p., 1993. Web.
Voordouw, G, Shen, Y, Harrington, C S, Teland, A J, Jack, T R, & Westlake, W S. Quantitative reverse sample genome probing of microbial communities and its application to oil field production waters. United States.
Voordouw, G, Shen, Y, Harrington, C S, Teland, A J, Jack, T R, and Westlake, W S. Wed . "Quantitative reverse sample genome probing of microbial communities and its application to oil field production waters". United States.
@article{osti_6703677,
title = {Quantitative reverse sample genome probing of microbial communities and its application to oil field production waters},
author = {Voordouw, G and Shen, Y and Harrington, C S and Teland, A J and Jack, T R and Westlake, W S},
abstractNote = {This paper presents a protocol for quantitative analysis of microbial communities by reverse sample genome probing is presented in which (i) whole community DNA is isolated and labeled in the presence of a known amount of an added internal standard and (ii) the resulting spiked reverse genome probe is hybridized with a master filter on which denatured genomic DNAs from bacterial standards isolated from the target environment were spotted in large amounts (up to 1,500 ng) in order to improve detection sensitivity. This protocol allowed reproducible fingerprinting of the microbial community in oil field production waters at 19 sites from which water and biofilm samples were collected. It appeared that selected sulfate-reducing bacteria were significantly enhanced in biofilms covering the metal surfaces in contact with the production waters.},
doi = {},
journal = {Applied and Environmental Microbiology; (United States)},
issn = {0099-2240},
number = ,
volume = 59:12,
place = {United States},
year = {1993},
month = {12}
}